In vitro clonal multilineage differentiation of distinct murine hematopoietic progenitor populations

Fatemeh Safi; Parashar Dhapola; Eva Erlandsson; Linda Geironson Ulfsson; Ariana S. Calderón; Charlotta Böiers; Göran Karlsson

doi:10.1016/j.xpro.2022.101965

In vitro clonal multilineage differentiation of distinct murine hematopoietic progenitor populations

Fatemeh Safi, Parashar Dhapola, Eva Erlandsson, Linda Geironson Ulfsson, Ariana S. Calderón, Charlotta Böiers, Göran Karlsson

Research output: Contribution to journal › Article › peer-review

Abstract

Here we describe an in vitro co-culture system that can differentiate hematopoietic progenitor populations to all major hematopoietic lineages at clonal level. We present both a sensitive single-cell switch-culture system as well as a less laborious alternative barcoding protocol more convenient for larger cell numbers. Importantly, generation of all lineages from single long-term hematopoietic stem cells are described, following 21 days of culture. This protocol represents an efficient tool for validation experiments for single-cell genomics data. For complete details on the use and execution of this protocol, please refer to Safi et al. (2022).¹

Original language	English
Article number	101965
Journal	STAR Protocols
Volume	4
Issue number	1
DOIs	https://doi.org/10.1016/j.xpro.2022.101965
Publication status	Published - 2023 Mar 17

Subject classification (UKÄ)

Cell and Molecular Biology

Free keywords

Bioinformatics
Cell Biology
Cell culture
Cell isolation
Flow Cytometry/Mass Cytometry
Genomics
Molecular Biology
Stem Cells

Access to Document

10.1016/j.xpro.2022.101965Licence: CC BY

1 Doctoral Thesis (compilation)

Early fate decisions in hematopoietic stem and progenitor cells. Through the lens of genomic and functional assays.
Safi, F., 2023, Lund: Lund University, Faculty of Medicine. 107 p.
Research output: Thesis › Doctoral Thesis (compilation)

Open Access
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abstract = "Here we describe an in vitro co-culture system that can differentiate hematopoietic progenitor populations to all major hematopoietic lineages at clonal level. We present both a sensitive single-cell switch-culture system as well as a less laborious alternative barcoding protocol more convenient for larger cell numbers. Importantly, generation of all lineages from single long-term hematopoietic stem cells are described, following 21 days of culture. This protocol represents an efficient tool for validation experiments for single-cell genomics data. For complete details on the use and execution of this protocol, please refer to Safi et al. (2022).1",

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T1 - In vitro clonal multilineage differentiation of distinct murine hematopoietic progenitor populations

AU - Safi, Fatemeh

AU - Dhapola, Parashar

AU - Erlandsson, Eva

AU - Ulfsson, Linda Geironson

AU - Calderón, Ariana S.

AU - Böiers, Charlotta

AU - Karlsson, Göran

PY - 2023/3/17

Y1 - 2023/3/17

N2 - Here we describe an in vitro co-culture system that can differentiate hematopoietic progenitor populations to all major hematopoietic lineages at clonal level. We present both a sensitive single-cell switch-culture system as well as a less laborious alternative barcoding protocol more convenient for larger cell numbers. Importantly, generation of all lineages from single long-term hematopoietic stem cells are described, following 21 days of culture. This protocol represents an efficient tool for validation experiments for single-cell genomics data. For complete details on the use and execution of this protocol, please refer to Safi et al. (2022).1

AB - Here we describe an in vitro co-culture system that can differentiate hematopoietic progenitor populations to all major hematopoietic lineages at clonal level. We present both a sensitive single-cell switch-culture system as well as a less laborious alternative barcoding protocol more convenient for larger cell numbers. Importantly, generation of all lineages from single long-term hematopoietic stem cells are described, following 21 days of culture. This protocol represents an efficient tool for validation experiments for single-cell genomics data. For complete details on the use and execution of this protocol, please refer to Safi et al. (2022).1

KW - Bioinformatics

KW - Cell Biology

KW - Cell culture

KW - Cell isolation

KW - Flow Cytometry/Mass Cytometry

KW - Genomics

KW - Molecular Biology

KW - Stem Cells

U2 - 10.1016/j.xpro.2022.101965

DO - 10.1016/j.xpro.2022.101965

M3 - Article

C2 - 36633951

AN - SCOPUS:85146080918

SN - 2666-1667

VL - 4

JO - STAR Protocols

JF - STAR Protocols

IS - 1

M1 - 101965

ER -

In vitro clonal multilineage differentiation of distinct murine hematopoietic progenitor populations

Abstract

Subject classification (UKÄ)

Free keywords

Access to Document

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Research output

Early fate decisions in hematopoietic stem and progenitor cells. Through the lens of genomic and functional assays.

Cite this