In vitro molecular evolution of antibody genes mimicking receptor revision

Peter Ellmark; O Esteban; Christina Furebring; Ann-Christin Malmborg Hager; Mats Ohlin

doi:10.1016/S0161-5890(02)00109-8

In vitro molecular evolution of antibody genes mimicking receptor revision

Peter Ellmark, O Esteban, Christina Furebring, Ann-Christin Malmborg Hager, Mats Ohlin

Department of Immunotechnology

Research output: Contribution to journal › Article › peer-review

Abstract

Antibody evolution in vivo proceeds mainly by stepwise improvements, accomplished by single base pair substitutions. Lately, receptor revision, i.e. exchange of large parts of the V gene for another sequence, has been suggested to provide a complementary route for affinity maturation. By employing a receptor revision like evolution process in vitro using combinatorial libraries and phage display selection, we demonstrate here that maturation of a clone may preferentially proceed through exchange of a large gene segment rather than via minor sequence changes. These modifications of a CD40-specific human antibody fragment outline how receptor revision like events may provide an advantage to a particular clonotype put under selective pressure. (C) 2002 Elsevier Science Ltd. All rights reserved.

Original language	English
Pages (from-to)	349-356
Journal	Molecular Immunology
Volume	39
Issue number	5-6
DOIs	https://doi.org/10.1016/S0161-5890(02)00109-8
Publication status	Published - 2002

Subject classification (UKÄ)

Immunology in the medical area

Free keywords

phage display
affinity maturation
antibody
receptor revision
somatic hypermutation

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10.1016/S0161-5890(02)00109-8

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title = "In vitro molecular evolution of antibody genes mimicking receptor revision",

abstract = "Antibody evolution in vivo proceeds mainly by stepwise improvements, accomplished by single base pair substitutions. Lately, receptor revision, i.e. exchange of large parts of the V gene for another sequence, has been suggested to provide a complementary route for affinity maturation. By employing a receptor revision like evolution process in vitro using combinatorial libraries and phage display selection, we demonstrate here that maturation of a clone may preferentially proceed through exchange of a large gene segment rather than via minor sequence changes. These modifications of a CD40-specific human antibody fragment outline how receptor revision like events may provide an advantage to a particular clonotype put under selective pressure. (C) 2002 Elsevier Science Ltd. All rights reserved.",

keywords = "phage display, affinity maturation, antibody, receptor revision, somatic hypermutation",

author = "Peter Ellmark and O Esteban and Christina Furebring and {Malmborg Hager}, Ann-Christin and Mats Ohlin",

year = "2002",

doi = "10.1016/S0161-5890(02)00109-8",

language = "English",

volume = "39",

pages = "349--356",

journal = "Molecular Immunology",

issn = "1872-9142",

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T1 - In vitro molecular evolution of antibody genes mimicking receptor revision

AU - Ellmark, Peter

AU - Esteban, O

AU - Furebring, Christina

AU - Malmborg Hager, Ann-Christin

AU - Ohlin, Mats

PY - 2002

Y1 - 2002

N2 - Antibody evolution in vivo proceeds mainly by stepwise improvements, accomplished by single base pair substitutions. Lately, receptor revision, i.e. exchange of large parts of the V gene for another sequence, has been suggested to provide a complementary route for affinity maturation. By employing a receptor revision like evolution process in vitro using combinatorial libraries and phage display selection, we demonstrate here that maturation of a clone may preferentially proceed through exchange of a large gene segment rather than via minor sequence changes. These modifications of a CD40-specific human antibody fragment outline how receptor revision like events may provide an advantage to a particular clonotype put under selective pressure. (C) 2002 Elsevier Science Ltd. All rights reserved.

AB - Antibody evolution in vivo proceeds mainly by stepwise improvements, accomplished by single base pair substitutions. Lately, receptor revision, i.e. exchange of large parts of the V gene for another sequence, has been suggested to provide a complementary route for affinity maturation. By employing a receptor revision like evolution process in vitro using combinatorial libraries and phage display selection, we demonstrate here that maturation of a clone may preferentially proceed through exchange of a large gene segment rather than via minor sequence changes. These modifications of a CD40-specific human antibody fragment outline how receptor revision like events may provide an advantage to a particular clonotype put under selective pressure. (C) 2002 Elsevier Science Ltd. All rights reserved.

KW - phage display

KW - affinity maturation

KW - antibody

KW - receptor revision

KW - somatic hypermutation

U2 - 10.1016/S0161-5890(02)00109-8

DO - 10.1016/S0161-5890(02)00109-8

M3 - Article

SN - 1872-9142

VL - 39

SP - 349

EP - 356

JO - Molecular Immunology

JF - Molecular Immunology

IS - 5-6

ER -

In vitro molecular evolution of antibody genes mimicking receptor revision

Abstract

Subject classification (UKÄ)

Free keywords

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