In vitro sliding of actin filaments labelled with single quantum dots

A Mansson, M Sundberg, M Balaz, Richard Bunk, I A Nicholls, Pär Omling, S Tagerud, Lars Montelius

Research output: Contribution to journalArticlepeer-review

125 Citations (SciVal)

Abstract

We recently refined the in vitro motility assay for studies of actomyosin function to achieve rectified myosin induced sliding of actin filaments. This paves the way, both for detailed functional studies of actomyosin and for nanotechnological applications. In the latter applications it would be desirable to use actin filaments for transportation of cargoes (e.g., enzymes) between different predetermined locations on a chip. We here describe how single quantum dot labelling of isolated actin filaments simultaneously provides handles for cargo attachment and bright and photostable fluorescence labels facilitating cargo detection and filament tracking. Labelling was achieved with preserved actomyosin function using streptavidin-coated CdSe quantum dots (Qdots). These nanocrystals have several unique physical properties and the present work describes their first use for functional studies of isolated proteins outside the cell. The results, in addition to the nanotechnology developments, open for new types of in vitro assays of isolated biomolecules. (C) 2003 Elsevier Inc. All rights reserved.
Original languageEnglish
Pages (from-to)529-534
JournalBiochemical and Biophysical Research Communications
Volume314
Issue number2
DOIs
Publication statusPublished - 2004

Subject classification (UKÄ)

  • Biological Sciences

Keywords

  • nanocrystal
  • nanostructure
  • quantum dot
  • myosin
  • actin
  • motility assay

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