@inbook{87707f6bcec74f70af0f5ab7cd4bd2d3,
title = "In vitro tumorigenic assay: colony forming assay for cancer stem cells",
abstract = "Colony forming or clonogenic assay is an in vitro quantitative technique to examine the capability of a single cell to grow into a large colony through clonal expansion. Clonogenic activity is a sensitive indicator of undifferentiated cancer stem cells. Here, we described the colony forming ability of the isolated breast cancer stem cells from the total population of cancer cells using double-layered, soft agarose-based assay. This method demonstrates that cancer stem cells can survive and generate colony growth in an anchorage-independent culture model. The 0.005% crystal violet solution is used in this assay to visualize the generated colonies.",
keywords = "Agarose, Cancer stem cell, Clonogenic assay, Colony growth, Crystal violet",
author = "Vijayalakshmi Rajendran and Jain, {Mayur Vilas}",
year = "2018",
doi = "10.1007/978-1-4939-7401-6_8",
language = "English",
volume = "1692",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "89--95",
booktitle = "Methods in Molecular Biology",
address = "United States",
}
