Influence of twenty potentially antiviral substances on in vitro multiplication of hepatitis A virus

Anders Widell; Bengt-Göran Hansson; Bo Öberg; Erik Nordenfelt

doi:10.1016/0166-3542(86)90030-6

Influence of twenty potentially antiviral substances on in vitro multiplication of hepatitis A virus

Anders Widell, Bengt-Göran Hansson, Bo Öberg, Erik Nordenfelt

Clinical Microbiology, Malmö

Research output: Contribution to journal › Article › peer-review

Abstract

A multiwell tissue culture system was developed to study the influence of various substances on hepatitis A virus (HAV) propagation. A panel of 20 substances of different structure types, each with known effect against at least some viruses, was studied at a concentration of 100 microM. Three substances showed reproducible inhibition. The strongest inhibitor, arabinosylcytosine, also produced cytotoxic changes in cells down to a concentration of 1 microM, and its effect was considered as nonspecific. Amantadine and ribavirin showed a moderate effect at 100 microM. A stronger inhibition was seen at 250 and 500 microM, doses that are toxic and impractical for clinical use. Although no promising candidates for antiviral treatment of hepatitis A have emerged from the present study, the assay model described here would seem useful in the screening of substances with inhibitory effects on HAV.

Original language	English
Pages (from-to)	103-112
Journal	Antiviral Research
Volume	6
Issue number	2
DOIs	https://doi.org/10.1016/0166-3542(86)90030-6
Publication status	Published - 1986

Subject classification (UKÄ)

Microbiology in the Medical Area

Free keywords

ribavirin
amantadine
arabinosylcytosine
hepatitis A

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/0166-3542(86)90030-6

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title = "Influence of twenty potentially antiviral substances on in vitro multiplication of hepatitis A virus",

abstract = "A multiwell tissue culture system was developed to study the influence of various substances on hepatitis A virus (HAV) propagation. A panel of 20 substances of different structure types, each with known effect against at least some viruses, was studied at a concentration of 100 microM. Three substances showed reproducible inhibition. The strongest inhibitor, arabinosylcytosine, also produced cytotoxic changes in cells down to a concentration of 1 microM, and its effect was considered as nonspecific. Amantadine and ribavirin showed a moderate effect at 100 microM. A stronger inhibition was seen at 250 and 500 microM, doses that are toxic and impractical for clinical use. Although no promising candidates for antiviral treatment of hepatitis A have emerged from the present study, the assay model described here would seem useful in the screening of substances with inhibitory effects on HAV.",

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doi = "10.1016/0166-3542(86)90030-6",

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T1 - Influence of twenty potentially antiviral substances on in vitro multiplication of hepatitis A virus

AU - Widell, Anders

AU - Hansson, Bengt-Göran

AU - Öberg, Bo

AU - Nordenfelt, Erik

PY - 1986

Y1 - 1986

N2 - A multiwell tissue culture system was developed to study the influence of various substances on hepatitis A virus (HAV) propagation. A panel of 20 substances of different structure types, each with known effect against at least some viruses, was studied at a concentration of 100 microM. Three substances showed reproducible inhibition. The strongest inhibitor, arabinosylcytosine, also produced cytotoxic changes in cells down to a concentration of 1 microM, and its effect was considered as nonspecific. Amantadine and ribavirin showed a moderate effect at 100 microM. A stronger inhibition was seen at 250 and 500 microM, doses that are toxic and impractical for clinical use. Although no promising candidates for antiviral treatment of hepatitis A have emerged from the present study, the assay model described here would seem useful in the screening of substances with inhibitory effects on HAV.

AB - A multiwell tissue culture system was developed to study the influence of various substances on hepatitis A virus (HAV) propagation. A panel of 20 substances of different structure types, each with known effect against at least some viruses, was studied at a concentration of 100 microM. Three substances showed reproducible inhibition. The strongest inhibitor, arabinosylcytosine, also produced cytotoxic changes in cells down to a concentration of 1 microM, and its effect was considered as nonspecific. Amantadine and ribavirin showed a moderate effect at 100 microM. A stronger inhibition was seen at 250 and 500 microM, doses that are toxic and impractical for clinical use. Although no promising candidates for antiviral treatment of hepatitis A have emerged from the present study, the assay model described here would seem useful in the screening of substances with inhibitory effects on HAV.

KW - ribavirin

KW - amantadine

KW - arabinosylcytosine

KW - hepatitis A

U2 - 10.1016/0166-3542(86)90030-6

DO - 10.1016/0166-3542(86)90030-6

M3 - Article

SN - 0166-3542

VL - 6

SP - 103

EP - 112

JO - Antiviral Research

JF - Antiviral Research

IS - 2

ER -

Influence of twenty potentially antiviral substances on in vitro multiplication of hepatitis A virus

Abstract

Subject classification (UKÄ)

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UN SDGs

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