Inhibition of splicing by serine-arginine rich protein 55 (SRp55) causes the appearance of partially spliced HIV-1 mRNAs in the cytoplasm

Anna Tranell, Susanne Tingsborg, Eva Maria Fenyö, Stefan Schwartz

Research output: Contribution to journalArticlepeer-review

9 Citations (SciVal)

Abstract

We have previously shown that SRp55 inhibits splicing from HIV-1 exon 3, thereby generating partially spliced mRNAs encoding HIV-1 vpr. Here we show that SRp55 also inhibits splicing from HIV-1 exon 5 to generate HIV-1 vpu/env mRNA, albeit with lower efficiency. We also show that inhibition of HIV-1 splicing by SRp55 causes the appearance of partially spliced vpu, env and vpr mRNAs in the cytoplasm. SRp55 could also induce production of extracellular p24gag from a rev-defective HIV-1 provirus. These results indicate that SRp55 aids in the generation of partially spliced and unspliced HIV-1 mRNAs. (C) 2011 Elsevier B.V. All rights reserved.
Original languageEnglish
Pages (from-to)82-91
JournalVirus Research
Volume157
Issue number1
DOIs
Publication statusPublished - 2011

Subject classification (UKÄ)

  • Microbiology in the medical area

Keywords

  • HIV-1
  • vpr
  • SRp55
  • Splicing

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