Integrated set up of sampling; sample treatment and assay of an intracellular protein during fermentation.

Mohammad Mazlomi, Martin Hedström, Bo Mattiasson

    Research output: Contribution to journalArticlepeer-review

    Abstract

    A complete system for on-line sampling, sample handling and analysis of fermentation processes has been set up. The system is designed such that a sample is taken from the fermentor, the metabolism of microbial cells is instantaneously arrested, the cells are lysed in order to release intracellular compounds and in a subsequent binding assay the content of target molecules is monitored. The system is constructed based on the flow-injection analysis concept. Good agreement between on-line analysis and off-line analysis is reported.
    Original languageEnglish
    Pages (from-to)366-371
    JournalJournal of Biotechnology
    Volume150
    Issue number3
    DOIs
    Publication statusPublished - 2010

    Subject classification (UKÄ)

    • Industrial Biotechnology

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