Interaction of sugars, polysaccharides and cells with boronate-containing copolymers: from solution to polymer brushes

Alexander Ivanov, Igor Galaev, Bo Mattiasson

Research output: Chapter in Book/Report/Conference proceedingPaper in conference proceedingpeer-review

39 Citations (SciVal)

Abstract

Interaction of mono- and disaccharides, polysaccharide particles and yeast cells with boronate-containing copolymers (BCC) of N-acryloyl-m-aminophenylboronic acid (NAAPBA) with NN-dimethylacrylamide (DMAA) or N-isopropylacrylamide (NIPAM) was studied. The binding of saccharides to BCC of NIPAM resulted in a shift of its phase transition temperature (Delta T-p), which provided a quantitative measure for the complex formation. Among the sugars typical of non-reducing ends of glycoproteins the Delta T-p decreased in the order: N-acetylneuraminic acid > xylose approximate to galactose > mannose approximate to fucose >> N-acetylglucosamine. Strong specific adsorption of the BCC on the cross-linked agarose gel Sepharose CL-6B (15-30 mg/ml gel at pH 9.2) was registered. The copolymers adsorption was due to boronate-sugar interactions and decreased with pH. Multivalent interaction of the BCC with the agarose gel has been proven by liquid column chromatography exhibiting a weak reversible adsorption of NAAPBA and almost irreversible adsorption of DMAA-NAAPBA copolymer from 0.1 M sodium phosphate buffer, pH 7.9. The two studied BCCs could be completely desorbed from the gel by 0.1 M fructose in aqueous buffered media with pH from 7.5 to 9.2. In turn, the agarose particles and yeast cells were found to adhere to siliceous supports end-grafted with boronate-BCC of N,N-dimethylacrylamide at pH >= 7.5, due to the actions. Quantitative detachment of adhered particles or cells could be attained by addition of 20 mM or 100 mM fructose, respectively, in the pH range from 7.5 to 9.2. Affinity adhesion of micron-size carbohydrate particles to boronate-containing polymer brushes fixed on solid supports was considered as a model system suggesting a new approach to isolation and separation of living cells. Copyright (c) 2006 John Wiley & Sons, Ltd.
Original languageEnglish
Title of host publicationJournal of Molecular Recognition
PublisherJohn Wiley & Sons Inc.
Pages322-331
Volume19
DOIs
Publication statusPublished - 2006
EventBioaffinity 2005 - Upsala, Sweden
Duration: 2005 Aug 142005 Aug 18

Publication series

Name
Number4
Volume19
ISSN (Print)1099-1352
ISSN (Electronic)0952-3499

Conference

ConferenceBioaffinity 2005
Country/TerritorySweden
CityUpsala
Period2005/08/142005/08/18

Subject classification (UKÄ)

  • Industrial Biotechnology

Keywords

  • thermoresponsive polymers
  • biomimetic
  • binding constant
  • boronic acid
  • cell adhesion
  • cell
  • oligosaccharides
  • chemisorption
  • detachment
  • immobilization

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