Interstitial photodynamic therapy - diagnostic measurements and treatment in rat malignant experimental tumours

M Stenberg; Marcelo Soto Thompson; Thomas Johansson; Sara Pålsson; C af Klinteberg; Stefan Andersson-Engels; U Stenram; Sune Svanberg; Katarina Svanberg

doi:10.1117/12.409325

Interstitial photodynamic therapy - diagnostic measurements and treatment in rat malignant experimental tumours

M Stenberg, Marcelo Soto Thompson, Thomas Johansson, Sara Pålsson, C af Klinteberg, Stefan Andersson-Engels, U Stenram, Sune Svanberg, Katarina Svanberg

Atomic Physics

Research output: Chapter in Book/Report/Conference proceeding › Paper in conference proceeding › peer-review

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Abstract

A recently developed multiple fibre system for treating malignant tumours with interstitial photodynamic therapy was used in studies on rats with colon adenocarcinoma inoculated into the muscles of the hind legs. The animals were intraperitonially administrated delta -aminolevulinic acid (ALA), which is metabolised to protoporphyrin IX (PpIX) in the tissue. The treatment system consists of a laser light source, a beam-splitting system dividing the light into three or six output fibres and a dosimetry programme calculating the optimal fibre position within the tumour as well as the treatment time needed to obtain a given threshold value of the light dose. One aim of the study was to compare the treatment outcome with the modelled dosimetry predictions. Tumour reduction was examined three days post treatment. A volume decrease was found in 85\% of the treated tumours. The mean volume reduction was 44\%, with one tumour completely disappearing. Histopathological examination three days post treatment showed substantial necrotic parts which, however, to a smaller extent were present also for non-treated tumours. These results indicated that the tumours have been under treated and the light dose has to be increased. Measurements of the build-up and photo-induced bleaching of PpIX using laser-induced fluorescence were also performed during the experiments.

Original language	English
Title of host publication	OPTICAL BIOPSY AND TISSUE OPTICS
Editors	IJ Bigio, GJ Muller, GJ Puppels, RW Steiner, Katarina Svanberg
Publisher	SPIE
Pages	151-157
Volume	4161
ISBN (Print)	0-8194-3817-0
DOIs	https://doi.org/10.1117/12.409325
Publication status	Published - 2000
Event	Conference on Optical Biopsy and Tissue Optics, 2000 - Amsterdam, Netherlands Duration: 2000 Jul 5 → 2000 Jul 6

Publication series

Name
Number	32
Volume	4161
ISSN (Print)	0277-786X
ISSN (Electronic)	1996-756X

Conference

Conference	Conference on Optical Biopsy and Tissue Optics, 2000
Country/Territory	Netherlands
City	Amsterdam
Period	2000/07/05 → 2000/07/06

Subject classification (UKÄ)

Atom and Molecular Physics and Optics

Access to Document

10.1117/12.409325

Stenberg2000

Cite this

Stenberg, M., Soto Thompson, M., Johansson, T., Pålsson, S., af Klinteberg, C., Andersson-Engels, S., Stenram, U., Svanberg, S., & Svanberg, K. (2000). Interstitial photodynamic therapy - diagnostic measurements and treatment in rat malignant experimental tumours. In IJ. Bigio, GJ. Muller, GJ. Puppels, RW. Steiner, & K. Svanberg (Eds.), OPTICAL BIOPSY AND TISSUE OPTICS (Vol. 4161, pp. 151-157). SPIE. https://doi.org/10.1117/12.409325

@inproceedings{f69a643e0ae7435e9c8e225d8dadc5f8,

title = "Interstitial photodynamic therapy - diagnostic measurements and treatment in rat malignant experimental tumours",

abstract = "A recently developed multiple fibre system for treating malignant tumours with interstitial photodynamic therapy was used in studies on rats with colon adenocarcinoma inoculated into the muscles of the hind legs. The animals were intraperitonially administrated delta -aminolevulinic acid (ALA), which is metabolised to protoporphyrin IX (PpIX) in the tissue. The treatment system consists of a laser light source, a beam-splitting system dividing the light into three or six output fibres and a dosimetry programme calculating the optimal fibre position within the tumour as well as the treatment time needed to obtain a given threshold value of the light dose. One aim of the study was to compare the treatment outcome with the modelled dosimetry predictions. Tumour reduction was examined three days post treatment. A volume decrease was found in 85\% of the treated tumours. The mean volume reduction was 44\%, with one tumour completely disappearing. Histopathological examination three days post treatment showed substantial necrotic parts which, however, to a smaller extent were present also for non-treated tumours. These results indicated that the tumours have been under treated and the light dose has to be increased. Measurements of the build-up and photo-induced bleaching of PpIX using laser-induced fluorescence were also performed during the experiments.",

author = "M Stenberg and {Soto Thompson}, Marcelo and Thomas Johansson and Sara P{\aa}lsson and {af Klinteberg}, C and Stefan Andersson-Engels and U Stenram and Sune Svanberg and Katarina Svanberg",

year = "2000",

doi = "10.1117/12.409325",

language = "English",

isbn = "0-8194-3817-0",

volume = "4161",

publisher = "SPIE",

number = "32",

pages = "151--157",

editor = "IJ Bigio and GJ Muller and GJ Puppels and RW Steiner and Katarina Svanberg",

booktitle = "OPTICAL BIOPSY AND TISSUE OPTICS",

address = "United States",

note = "Conference on Optical Biopsy and Tissue Optics, 2000 ; Conference date: 05-07-2000 Through 06-07-2000",

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Stenberg, M, Soto Thompson, M, Johansson, T, Pålsson, S, af Klinteberg, C, Andersson-Engels, S, Stenram, U, Svanberg, S & Svanberg, K 2000, Interstitial photodynamic therapy - diagnostic measurements and treatment in rat malignant experimental tumours. in IJ Bigio, GJ Muller, GJ Puppels, RW Steiner & K Svanberg (eds), OPTICAL BIOPSY AND TISSUE OPTICS. vol. 4161, SPIE, pp. 151-157, Conference on Optical Biopsy and Tissue Optics, 2000, Amsterdam, Netherlands, 2000/07/05. https://doi.org/10.1117/12.409325

Interstitial photodynamic therapy - diagnostic measurements and treatment in rat malignant experimental tumours. / Stenberg, M; Soto Thompson, Marcelo; Johansson, Thomas et al.
OPTICAL BIOPSY AND TISSUE OPTICS. ed. / IJ Bigio; GJ Muller; GJ Puppels; RW Steiner; Katarina Svanberg. Vol. 4161 SPIE, 2000. p. 151-157.

Research output: Chapter in Book/Report/Conference proceeding › Paper in conference proceeding › peer-review

TY - GEN

T1 - Interstitial photodynamic therapy - diagnostic measurements and treatment in rat malignant experimental tumours

AU - Stenberg, M

AU - Soto Thompson, Marcelo

AU - Johansson, Thomas

AU - Pålsson, Sara

AU - af Klinteberg, C

AU - Andersson-Engels, Stefan

AU - Stenram, U

AU - Svanberg, Sune

AU - Svanberg, Katarina

PY - 2000

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N2 - A recently developed multiple fibre system for treating malignant tumours with interstitial photodynamic therapy was used in studies on rats with colon adenocarcinoma inoculated into the muscles of the hind legs. The animals were intraperitonially administrated delta -aminolevulinic acid (ALA), which is metabolised to protoporphyrin IX (PpIX) in the tissue. The treatment system consists of a laser light source, a beam-splitting system dividing the light into three or six output fibres and a dosimetry programme calculating the optimal fibre position within the tumour as well as the treatment time needed to obtain a given threshold value of the light dose. One aim of the study was to compare the treatment outcome with the modelled dosimetry predictions. Tumour reduction was examined three days post treatment. A volume decrease was found in 85\% of the treated tumours. The mean volume reduction was 44\%, with one tumour completely disappearing. Histopathological examination three days post treatment showed substantial necrotic parts which, however, to a smaller extent were present also for non-treated tumours. These results indicated that the tumours have been under treated and the light dose has to be increased. Measurements of the build-up and photo-induced bleaching of PpIX using laser-induced fluorescence were also performed during the experiments.

AB - A recently developed multiple fibre system for treating malignant tumours with interstitial photodynamic therapy was used in studies on rats with colon adenocarcinoma inoculated into the muscles of the hind legs. The animals were intraperitonially administrated delta -aminolevulinic acid (ALA), which is metabolised to protoporphyrin IX (PpIX) in the tissue. The treatment system consists of a laser light source, a beam-splitting system dividing the light into three or six output fibres and a dosimetry programme calculating the optimal fibre position within the tumour as well as the treatment time needed to obtain a given threshold value of the light dose. One aim of the study was to compare the treatment outcome with the modelled dosimetry predictions. Tumour reduction was examined three days post treatment. A volume decrease was found in 85\% of the treated tumours. The mean volume reduction was 44\%, with one tumour completely disappearing. Histopathological examination three days post treatment showed substantial necrotic parts which, however, to a smaller extent were present also for non-treated tumours. These results indicated that the tumours have been under treated and the light dose has to be increased. Measurements of the build-up and photo-induced bleaching of PpIX using laser-induced fluorescence were also performed during the experiments.

U2 - 10.1117/12.409325

DO - 10.1117/12.409325

M3 - Paper in conference proceeding

SN - 0-8194-3817-0

VL - 4161

SP - 151

EP - 157

BT - OPTICAL BIOPSY AND TISSUE OPTICS

A2 - Bigio, IJ

A2 - Muller, GJ

A2 - Puppels, GJ

A2 - Steiner, RW

A2 - Svanberg, Katarina

PB - SPIE

T2 - Conference on Optical Biopsy and Tissue Optics, 2000

Y2 - 5 July 2000 through 6 July 2000

ER -

Interstitial photodynamic therapy - diagnostic measurements and treatment in rat malignant experimental tumours

Abstract

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