Intra-individual variation in sperm chromatin structure assay parameters in men from infertile couples: clinical implications.

Juris Erenpreiss, Mona Bungum, M Spano, Saad Elzanaty, J Orbidans, Aleksander Giwercman

Research output: Contribution to journalArticlepeer-review

55 Citations (SciVal)

Abstract

BACKGROUND: Sperm DNA integrity is an important factor in the prognosis of male fertility. In this study, we investigated intra-individual variation of sperm chromatin structure assay (SCSA) parameters in infertility patients undergoing assisted reproductive techniques (ARTs). METHODS: Retrospective study of 282 consecutive patients referred for ART [intrauterine insemination (IUI), IVF or ICSI] with repeated (between 2 and 5) SCSA measurements. RESULTS: Mean coefficient of variation (CV) of DNA Fragmentation Index (DFI) for repeated SCSA measurements was 29%. A high proportion [37%; 95% confidence interval (CI): 27%, 49%] of patients with DFI > 30% in the first test had DFI < 30% in the second test. Also, a considerable proportion (27%; 95% CI : 16%, 40%) of patients with 21-30% DFI values in the first test had DFI > 30% in the second test. CONCLUSIONS: Intra-individual variability in DFI is significant, therefore repeated SCSA measurements are recommended. The biological mechanisms behind these variations remain to be elucidated.
Original languageEnglish
Pages (from-to)2061-2064
JournalHuman Reproduction
Volume21
Issue number2006 May 9
DOIs
Publication statusPublished - 2006

Subject classification (UKÄ)

  • Clinical Medicine

Keywords

  • flow cytometry
  • sperm DNA integrity
  • SCSA
  • infertility
  • sperm chromatin

Fingerprint

Dive into the research topics of 'Intra-individual variation in sperm chromatin structure assay parameters in men from infertile couples: clinical implications.'. Together they form a unique fingerprint.

Cite this