Investigation of sera from various species by using lectin affinity arrays and scanning ellipsometry

J Carlsson; M Mecklenburg; I Lundström; Bengt Danielsson; F Winquist

doi:10.1016/j.aca.2004.09.022

Investigation of sera from various species by using lectin affinity arrays and scanning ellipsometry

J Carlsson, M Mecklenburg, I Lundström, Bengt Danielsson, F Winquist

Pure and Applied Biochemistry

Research output: Contribution to journal › Article › peer-review

Abstract

Serum proteins of different species and of different human blood groups exhibit various protein glycosylation patterns. Sera from human, pig, sheep and guinea pig have been applied to a panel of eight different lectins immobilized on a gold wafer. The biorecognition has been evaluated with scanning ellipsometry and the two-dimensional matrices obtained have been treated with image analysis and MVDA for evaluation. The results showed a clear difference in protein binding pattern between the different species and thereby separation of the different sera could be made. Dendograms indicate that human and pig sera are the most related of the four different sera investigated.

Original language	English
Pages (from-to)	167-171
Journal	Analytica Chimica Acta
Volume	530
Issue number	2
DOIs	https://doi.org/10.1016/j.aca.2004.09.022
Publication status	Published - 2005

Subject classification (UKÄ)

Analytical Chemistry

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10.1016/j.aca.2004.09.022

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title = "Investigation of sera from various species by using lectin affinity arrays and scanning ellipsometry",

abstract = "Serum proteins of different species and of different human blood groups exhibit various protein glycosylation patterns. Sera from human, pig, sheep and guinea pig have been applied to a panel of eight different lectins immobilized on a gold wafer. The biorecognition has been evaluated with scanning ellipsometry and the two-dimensional matrices obtained have been treated with image analysis and MVDA for evaluation. The results showed a clear difference in protein binding pattern between the different species and thereby separation of the different sera could be made. Dendograms indicate that human and pig sera are the most related of the four different sera investigated.",

author = "J Carlsson and M Mecklenburg and I Lundstr{\"o}m and Bengt Danielsson and F Winquist",

year = "2005",

doi = "10.1016/j.aca.2004.09.022",

language = "English",

volume = "530",

pages = "167--171",

journal = "Analytica Chimica Acta",

issn = "1873-4324",

publisher = "Elsevier",

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TY - JOUR

T1 - Investigation of sera from various species by using lectin affinity arrays and scanning ellipsometry

AU - Carlsson, J

AU - Mecklenburg, M

AU - Lundström, I

AU - Danielsson, Bengt

AU - Winquist, F

PY - 2005

Y1 - 2005

N2 - Serum proteins of different species and of different human blood groups exhibit various protein glycosylation patterns. Sera from human, pig, sheep and guinea pig have been applied to a panel of eight different lectins immobilized on a gold wafer. The biorecognition has been evaluated with scanning ellipsometry and the two-dimensional matrices obtained have been treated with image analysis and MVDA for evaluation. The results showed a clear difference in protein binding pattern between the different species and thereby separation of the different sera could be made. Dendograms indicate that human and pig sera are the most related of the four different sera investigated.

AB - Serum proteins of different species and of different human blood groups exhibit various protein glycosylation patterns. Sera from human, pig, sheep and guinea pig have been applied to a panel of eight different lectins immobilized on a gold wafer. The biorecognition has been evaluated with scanning ellipsometry and the two-dimensional matrices obtained have been treated with image analysis and MVDA for evaluation. The results showed a clear difference in protein binding pattern between the different species and thereby separation of the different sera could be made. Dendograms indicate that human and pig sera are the most related of the four different sera investigated.

U2 - 10.1016/j.aca.2004.09.022

DO - 10.1016/j.aca.2004.09.022

M3 - Article

SN - 1873-4324

VL - 530

SP - 167

EP - 171

JO - Analytica Chimica Acta

JF - Analytica Chimica Acta

IS - 2

ER -

Investigation of sera from various species by using lectin affinity arrays and scanning ellipsometry

Abstract

Subject classification (UKÄ)

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