Ion exchange chromatography of glucosamine and galactosamine in microgram amounts with quantitative determination and specific radioactivity assay

Stefan Lohmander

doi:10.1016/0304-4165(72)90003-7

Ion exchange chromatography of glucosamine and galactosamine in microgram amounts with quantitative determination and specific radioactivity assay

Stefan Lohmander

Karolinska Institute

Research output: Contribution to journal › Article › peer-review

Abstract

A rapid method for the separation and quantitative determination, including radioassay, of glucosamine and galactosamine in microgram amounts is described. It is based upon the use of a column of Aminex A-5 ion exchange resin eluted with a phosphate buffer at pH 7.00 and 60 °C. From each fraction half the volume is used for a scaled down Elson-Morgan procedure and other half for liquid scintillation counting. Amounts of about 0.01-1 μmole of hexosamine may be separated and determined.

Original language	English
Pages (from-to)	411-417
Number of pages	7
Journal	Biochimica et Biophysica Acta. General Subjects
Volume	264
Issue number	3
DOIs	https://doi.org/10.1016/0304-4165(72)90003-7
Publication status	Published - 1972 May 16

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10.1016/0304-4165(72)90003-7

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title = "Ion exchange chromatography of glucosamine and galactosamine in microgram amounts with quantitative determination and specific radioactivity assay",

abstract = "A rapid method for the separation and quantitative determination, including radioassay, of glucosamine and galactosamine in microgram amounts is described. It is based upon the use of a column of Aminex A-5 ion exchange resin eluted with a phosphate buffer at pH 7.00 and 60 °C. From each fraction half the volume is used for a scaled down Elson-Morgan procedure and other half for liquid scintillation counting. Amounts of about 0.01-1 μmole of hexosamine may be separated and determined.",

author = "Stefan Lohmander",

year = "1972",

month = may,

day = "16",

doi = "10.1016/0304-4165(72)90003-7",

language = "English",

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pages = "411--417",

journal = "Biochimica et Biophysica Acta. General Subjects",

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publisher = "Elsevier",

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T1 - Ion exchange chromatography of glucosamine and galactosamine in microgram amounts with quantitative determination and specific radioactivity assay

AU - Lohmander, Stefan

PY - 1972/5/16

Y1 - 1972/5/16

N2 - A rapid method for the separation and quantitative determination, including radioassay, of glucosamine and galactosamine in microgram amounts is described. It is based upon the use of a column of Aminex A-5 ion exchange resin eluted with a phosphate buffer at pH 7.00 and 60 °C. From each fraction half the volume is used for a scaled down Elson-Morgan procedure and other half for liquid scintillation counting. Amounts of about 0.01-1 μmole of hexosamine may be separated and determined.

AB - A rapid method for the separation and quantitative determination, including radioassay, of glucosamine and galactosamine in microgram amounts is described. It is based upon the use of a column of Aminex A-5 ion exchange resin eluted with a phosphate buffer at pH 7.00 and 60 °C. From each fraction half the volume is used for a scaled down Elson-Morgan procedure and other half for liquid scintillation counting. Amounts of about 0.01-1 μmole of hexosamine may be separated and determined.

UR - https://www.scopus.com/pages/publications/0015512384

U2 - 10.1016/0304-4165(72)90003-7

DO - 10.1016/0304-4165(72)90003-7

M3 - Article

C2 - 5027625

AN - SCOPUS:0015512384

SN - 0304-4165

VL - 264

SP - 411

EP - 417

JO - Biochimica et Biophysica Acta. General Subjects

JF - Biochimica et Biophysica Acta. General Subjects

IS - 3

ER -

Ion exchange chromatography of glucosamine and galactosamine in microgram amounts with quantitative determination and specific radioactivity assay

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