Isolation and Characterization of Shiga Toxin-Associated Microvesicles

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Abstract

Microvesicles are shed from cell surfaces during infectious or inflammatory conditions and may contribute to the pathogenesis of disease. During Shiga toxin-producing Escherichia coli (STEC) infection, microvesicles are released from blood cells. These microvesicles play a part in inflammation, thrombosis, hemolysis, and the transfer of the main virulence factor of STEC strains, Shiga toxin, to target organ cells. This chapter describes how to isolate blood cell- and cell culture-derived microvesicles from plasma or cell culture medium, respectively, and how to characterize these microvesicles by various methods, with special focus on Shiga toxin-associated microvesicles.

Original languageEnglish
Title of host publicationShiga Toxin-Producing E. coli
PublisherHumana Press
Pages207-228
Number of pages22
ISBN (Print)978-1-0716-1339-9
DOIs
Publication statusPublished - 2021

Publication series

NameMethods in Molecular Biology
Volume2291
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Subject classification (UKÄ)

  • Pediatrics
  • Cell and Molecular Biology

Keywords

  • Cells
  • Electron microscopy
  • Enzyme-linked immunosorbent assay (ELISA)
  • Extracellular vesicles
  • Flow cytometry
  • Live cell imaging
  • Microvesicles
  • Nanoparticle tracking analysis
  • Proteomics
  • Shiga toxin

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