Isolation and Characterization of Shiga Toxin-Associated Microvesicles

Annie Willysson; Anne lie Ståhl; Diana Karpman

doi:10.1007/978-1-0716-1339-9_9

Isolation and Characterization of Shiga Toxin-Associated Microvesicles

Annie Willysson, Anne lie Ståhl, Diana Karpman

Pediatric Nephrology

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Abstract

Microvesicles are shed from cell surfaces during infectious or inflammatory conditions and may contribute to the pathogenesis of disease. During Shiga toxin-producing Escherichia coli (STEC) infection, microvesicles are released from blood cells. These microvesicles play a part in inflammation, thrombosis, hemolysis, and the transfer of the main virulence factor of STEC strains, Shiga toxin, to target organ cells. This chapter describes how to isolate blood cell- and cell culture-derived microvesicles from plasma or cell culture medium, respectively, and how to characterize these microvesicles by various methods, with special focus on Shiga toxin-associated microvesicles.

Original language	English
Title of host publication	Shiga Toxin-Producing E. coli
Publisher	Humana Press
Pages	207-228
Number of pages	22
ISBN (Print)	978-1-0716-1339-9
DOIs	https://doi.org/10.1007/978-1-0716-1339-9_9
Publication status	Published - 2021

Publication series

Name	Methods in Molecular Biology
Volume	2291
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Subject classification (UKÄ)

Pediatrics
Cell and Molecular Biology

Keywords

Cells
Electron microscopy
Enzyme-linked immunosorbent assay (ELISA)
Extracellular vesicles
Flow cytometry
Live cell imaging
Microvesicles
Nanoparticle tracking analysis
Proteomics
Shiga toxin

Access to Document

10.1007/978-1-0716-1339-9_9

1 Doctoral Thesis (compilation)

Extracellular vesicles in Shiga toxin-mediated disease
Willysson, A., 2021, Lund : Lund University, Faculty of Medicine. 90 p.
Research output: Thesis › Doctoral Thesis (compilation)

Open Access
File

Cite this

@inbook{19c8e2f8fef84b4fbd98321fefe6a3aa,

title = "Isolation and Characterization of Shiga Toxin-Associated Microvesicles",

abstract = "Microvesicles are shed from cell surfaces during infectious or inflammatory conditions and may contribute to the pathogenesis of disease. During Shiga toxin-producing Escherichia coli (STEC) infection, microvesicles are released from blood cells. These microvesicles play a part in inflammation, thrombosis, hemolysis, and the transfer of the main virulence factor of STEC strains, Shiga toxin, to target organ cells. This chapter describes how to isolate blood cell- and cell culture-derived microvesicles from plasma or cell culture medium, respectively, and how to characterize these microvesicles by various methods, with special focus on Shiga toxin-associated microvesicles.",

keywords = "Cells, Electron microscopy, Enzyme-linked immunosorbent assay (ELISA), Extracellular vesicles, Flow cytometry, Live cell imaging, Microvesicles, Nanoparticle tracking analysis, Proteomics, Shiga toxin",

author = "Annie Willysson and St{\aa}hl, {Anne lie} and Diana Karpman",

year = "2021",

doi = "10.1007/978-1-0716-1339-9_9",

language = "English",

isbn = "978-1-0716-1339-9",

series = "Methods in Molecular Biology",

publisher = "Humana Press",

pages = "207--228",

booktitle = "Shiga Toxin-Producing E. coli",

address = "United States",

}

TY - CHAP

T1 - Isolation and Characterization of Shiga Toxin-Associated Microvesicles

AU - Willysson, Annie

AU - Ståhl, Anne lie

AU - Karpman, Diana

PY - 2021

Y1 - 2021

N2 - Microvesicles are shed from cell surfaces during infectious or inflammatory conditions and may contribute to the pathogenesis of disease. During Shiga toxin-producing Escherichia coli (STEC) infection, microvesicles are released from blood cells. These microvesicles play a part in inflammation, thrombosis, hemolysis, and the transfer of the main virulence factor of STEC strains, Shiga toxin, to target organ cells. This chapter describes how to isolate blood cell- and cell culture-derived microvesicles from plasma or cell culture medium, respectively, and how to characterize these microvesicles by various methods, with special focus on Shiga toxin-associated microvesicles.

AB - Microvesicles are shed from cell surfaces during infectious or inflammatory conditions and may contribute to the pathogenesis of disease. During Shiga toxin-producing Escherichia coli (STEC) infection, microvesicles are released from blood cells. These microvesicles play a part in inflammation, thrombosis, hemolysis, and the transfer of the main virulence factor of STEC strains, Shiga toxin, to target organ cells. This chapter describes how to isolate blood cell- and cell culture-derived microvesicles from plasma or cell culture medium, respectively, and how to characterize these microvesicles by various methods, with special focus on Shiga toxin-associated microvesicles.

KW - Cells

KW - Electron microscopy

KW - Enzyme-linked immunosorbent assay (ELISA)

KW - Extracellular vesicles

KW - Flow cytometry

KW - Live cell imaging

KW - Microvesicles

KW - Nanoparticle tracking analysis

KW - Proteomics

KW - Shiga toxin

U2 - 10.1007/978-1-0716-1339-9_9

DO - 10.1007/978-1-0716-1339-9_9

M3 - Book chapter

C2 - 33704755

AN - SCOPUS:85102845966

SN - 978-1-0716-1339-9

T3 - Methods in Molecular Biology

SP - 207

EP - 228

BT - Shiga Toxin-Producing E. coli

PB - Humana Press

ER -

Isolation and Characterization of Shiga Toxin-Associated Microvesicles

Abstract

Publication series

Subject classification (UKÄ)

Keywords

Access to Document

Fingerprint

Research output

Extracellular vesicles in Shiga toxin-mediated disease

Cite this