Research output per year
Research output per year
Annie Willysson, Anne lie Ståhl, Diana Karpman
Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review
Microvesicles are shed from cell surfaces during infectious or inflammatory conditions and may contribute to the pathogenesis of disease. During Shiga toxin-producing Escherichia coli (STEC) infection, microvesicles are released from blood cells. These microvesicles play a part in inflammation, thrombosis, hemolysis, and the transfer of the main virulence factor of STEC strains, Shiga toxin, to target organ cells. This chapter describes how to isolate blood cell- and cell culture-derived microvesicles from plasma or cell culture medium, respectively, and how to characterize these microvesicles by various methods, with special focus on Shiga toxin-associated microvesicles.
Original language | English |
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Title of host publication | Shiga Toxin-Producing E. coli |
Publisher | Humana Press |
Pages | 207-228 |
Number of pages | 22 |
ISBN (Print) | 978-1-0716-1339-9 |
DOIs | |
Publication status | Published - 2021 |
Name | Methods in Molecular Biology |
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Volume | 2291 |
ISSN (Print) | 1064-3745 |
ISSN (Electronic) | 1940-6029 |
Research output: Thesis › Doctoral Thesis (compilation)