Isolation and identification from Salvia officinalis of two diterpenes which inhibit t-butylbicyclophosphoro[35S]thionate binding to chloride channel of rat cerebrocortical membranes in vitro

Diane M Rutherford; Mogens P Nielsen; Susanne K Hansen; Michael-Robin Witt; Ola Bergendorff; Olov Sterner

doi:10.1016/0304-3940(92)90441-9

Isolation and identification from Salvia officinalis of two diterpenes which inhibit t-butylbicyclophosphoro[35S]thionate binding to chloride channel of rat cerebrocortical membranes in vitro

Diane M Rutherford, Mogens P Nielsen, Susanne K Hansen, Michael-Robin Witt, Ola Bergendorff, Olov Sterner

Research output: Contribution to journal › Article › peer-review

Abstract

Ethanolic extracts from dried leaves of sage (Salvia officinalis) showed inhibition of [35S]tertiary-butylbicyclophosphorothionate ([35S]TBPS) binding to rat brain membranes in vitro. This ligand is considered to bind to the chloride channel of the GABA/benzodiazepine receptor complex in brain tissue. Substances having inhibitory activity were purified and their chemical structure identified as the diterpenes carnosic acid and carnosol (IC50 values of 33 +/- 3 microM and 57 +/- 4 microM, respectively). The two compounds did not affect binding of the ligands [3H]muscimol and [3H]diazepam to the GABA/benzodiazepine complex in vitro. Saturation experiments of [35S]TBPS binding indicated that carnosic acid decreases the binding affinity.

Original language	English
Pages (from-to)	224-226
Journal	Neuroscience Letters
Volume	135
Issue number	2
DOIs	https://doi.org/10.1016/0304-3940(92)90441-9
Publication status	Published - 1992

Bibliographical note

The information about affiliations in this record was updated in December 2015.
The record was previously connected to the following departments: Department of Dermatology and Venereology (Lund) (013006000), Organic chemistry (S/LTH) (011001240)

Subject classification (UKÄ)

Neurosciences

Free keywords

γ-Aminobutyric acid
Benzodiazepine receptor
[35S]TBPS binding
Salvia officinalis
Diterpenoid
Carnosol
Carnosic acid

Access to Document

10.1016/0304-3940(92)90441-9

Cite this

Rutherford, D. M., Nielsen, M. P., Hansen, S. K., Witt, M-R., Bergendorff, O., & Sterner, O. (1992). Isolation and identification from Salvia officinalis of two diterpenes which inhibit t-butylbicyclophosphoro[35S]thionate binding to chloride channel of rat cerebrocortical membranes in vitro. Neuroscience Letters, 135(2), 224-226. https://doi.org/10.1016/0304-3940(92)90441-9

@article{8c26a01a40044fb3bbe189bf91155ca7,

title = "Isolation and identification from Salvia officinalis of two diterpenes which inhibit t-butylbicyclophosphoro[35S]thionate binding to chloride channel of rat cerebrocortical membranes in vitro",

abstract = "Ethanolic extracts from dried leaves of sage (Salvia officinalis) showed inhibition of [35S]tertiary-butylbicyclophosphorothionate ([35S]TBPS) binding to rat brain membranes in vitro. This ligand is considered to bind to the chloride channel of the GABA/benzodiazepine receptor complex in brain tissue. Substances having inhibitory activity were purified and their chemical structure identified as the diterpenes carnosic acid and carnosol (IC50 values of 33 +/- 3 microM and 57 +/- 4 microM, respectively). The two compounds did not affect binding of the ligands [3H]muscimol and [3H]diazepam to the GABA/benzodiazepine complex in vitro. Saturation experiments of [35S]TBPS binding indicated that carnosic acid decreases the binding affinity.",

keywords = "γ-Aminobutyric acid, Benzodiazepine receptor, [35S]TBPS binding, Salvia officinalis, Diterpenoid, Carnosol, Carnosic acid",

author = "Rutherford, {Diane M} and Nielsen, {Mogens P} and Hansen, {Susanne K} and Michael-Robin Witt and Ola Bergendorff and Olov Sterner",

note = "The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Department of Dermatology and Venereology (Lund) (013006000), Organic chemistry (S/LTH) (011001240)",

year = "1992",

doi = "10.1016/0304-3940(92)90441-9",

language = "English",

volume = "135",

pages = "224--226",

journal = "Neuroscience Letters",

issn = "0304-3940",

publisher = "Elsevier",

number = "2",

}

Rutherford, DM, Nielsen, MP, Hansen, SK, Witt, M-R, Bergendorff, O & Sterner, O 1992, 'Isolation and identification from Salvia officinalis of two diterpenes which inhibit t-butylbicyclophosphoro[35S]thionate binding to chloride channel of rat cerebrocortical membranes in vitro', Neuroscience Letters, vol. 135, no. 2, pp. 224-226. https://doi.org/10.1016/0304-3940(92)90441-9

Isolation and identification from Salvia officinalis of two diterpenes which inhibit t-butylbicyclophosphoro[35S]thionate binding to chloride channel of rat cerebrocortical membranes in vitro. / Rutherford, Diane M; Nielsen, Mogens P; Hansen, Susanne K et al.

In: Neuroscience Letters, Vol. 135, No. 2, 1992, p. 224-226.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Isolation and identification from Salvia officinalis of two diterpenes which inhibit t-butylbicyclophosphoro[35S]thionate binding to chloride channel of rat cerebrocortical membranes in vitro

AU - Rutherford, Diane M

AU - Nielsen, Mogens P

AU - Hansen, Susanne K

AU - Witt, Michael-Robin

AU - Bergendorff, Ola

AU - Sterner, Olov

N1 - The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Department of Dermatology and Venereology (Lund) (013006000), Organic chemistry (S/LTH) (011001240)

PY - 1992

Y1 - 1992

N2 - Ethanolic extracts from dried leaves of sage (Salvia officinalis) showed inhibition of [35S]tertiary-butylbicyclophosphorothionate ([35S]TBPS) binding to rat brain membranes in vitro. This ligand is considered to bind to the chloride channel of the GABA/benzodiazepine receptor complex in brain tissue. Substances having inhibitory activity were purified and their chemical structure identified as the diterpenes carnosic acid and carnosol (IC50 values of 33 +/- 3 microM and 57 +/- 4 microM, respectively). The two compounds did not affect binding of the ligands [3H]muscimol and [3H]diazepam to the GABA/benzodiazepine complex in vitro. Saturation experiments of [35S]TBPS binding indicated that carnosic acid decreases the binding affinity.

AB - Ethanolic extracts from dried leaves of sage (Salvia officinalis) showed inhibition of [35S]tertiary-butylbicyclophosphorothionate ([35S]TBPS) binding to rat brain membranes in vitro. This ligand is considered to bind to the chloride channel of the GABA/benzodiazepine receptor complex in brain tissue. Substances having inhibitory activity were purified and their chemical structure identified as the diterpenes carnosic acid and carnosol (IC50 values of 33 +/- 3 microM and 57 +/- 4 microM, respectively). The two compounds did not affect binding of the ligands [3H]muscimol and [3H]diazepam to the GABA/benzodiazepine complex in vitro. Saturation experiments of [35S]TBPS binding indicated that carnosic acid decreases the binding affinity.

KW - γ-Aminobutyric acid

KW - Benzodiazepine receptor

KW - [35S]TBPS binding

KW - Salvia officinalis

KW - Diterpenoid

KW - Carnosol

KW - Carnosic acid

U2 - 10.1016/0304-3940(92)90441-9

DO - 10.1016/0304-3940(92)90441-9

M3 - Article

SN - 0304-3940

VL - 135

SP - 224

EP - 226

JO - Neuroscience Letters

JF - Neuroscience Letters

IS - 2

ER -

Isolation and identification from Salvia officinalis of two diterpenes which inhibit t-butylbicyclophosphoro[35S]thionate binding to chloride channel of rat cerebrocortical membranes in vitro

Abstract

Bibliographical note

Subject classification (UKÄ)

Free keywords

Access to Document

Fingerprint

Cite this