Kinetic analysis of amyloid formation

Georg Meisl, Thomas C.T. Michaels, Sara Linse, Tuomas P.J. Knowles

Research output: Chapter in Book/Report/Conference proceedingBook chapterpeer-review

10 Citations (SciVal)

Abstract

The formation of amyloid fibrils is a central phenomenon in the progressive pathology of many neurodegenerative diseases, as well as in the fabrication of functional materials. Several different molecular processes acting in concert are responsible for the formation of amyloid fibrils from monomeric protein in solution. Here, we describe a method to determine which microscopic processes drive the overall formation of fibrils by using chemical kinetics in combination with systematic experimental datasets analysed in a global manner. We outline general concepts for obtaining suitable kinetic data and detail the key stages of data analysis, from quality control to the verification of a specific mechanism of aggregation.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press
Pages181-196
Number of pages16
DOIs
Publication statusPublished - 2018 Jan 1

Publication series

NameMethods in Molecular Biology
Volume1779
ISSN (Print)1064-3745

Subject classification (UKÄ)

  • Biochemistry and Molecular Biology

Keywords

  • Amyloid fibrils
  • Kinetic modeling
  • Molecular-level mechanism
  • Protein aggregation
  • Self-assembly

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