Leukotriene D4-induced calcium signaling in human intestinal epithelial cells

Charles Kumar Thodeti, Anita Sjölander

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review


LTD4 induces a calcium signal that consists of a mobilization from internal stores regulated by PTX-insensitive G protein, Rho, and, an influx across the plasma membrane regulated by PTX-sensitive Gi protein in human intestinal epithelial cells. The LTD4 induced mobilization of Ca2+ is mediated by a PH domain dependent association between PLC-gamma 1 and G beta gamma subunits. This interaction requires Src kinase activity as well as the association of this kinase with PLC-gamma 1, suggesting a G beta gamma mediated recruitment of proteins to the plasma membrane and formation of a signaling complex which is essential for the downstream Ca2+ signal. We found a cAMP-dependent protein kinase activity upstream of tyrosine kinase(s) and downstream of Gi protein, that is essential for LTD4-induced Ca2+ influx. This model of the LTD4-induced Ca2+ signaling pathways in intestinal epithelial cells is outlined schematically in Fig. 1.

Original languageEnglish
Title of host publicationEicosanoids and other bioactive Lipids in Cancer, Inflammation, and Radiation Injury, 5
EditorsK Honn, L Marnett, S Nigam, E Dennis, C Serhan
ISBN (Electronic)978-1-4615-0193-0
Publication statusPublished - 2002

Publication series

NameAdvances in Experimental Medicine and Biology
ISSN (Print)0065-2598

Subject classification (UKÄ)

  • Cell and Molecular Biology

Free keywords

  • Calcium Signaling/drug effects
  • Humans
  • Intestinal Mucosa/drug effects
  • Leukotriene D4/pharmacology


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