Abstract
Rhizopus arrhizus lipase immobilized on celite was used to prepare isomerically pure 2-monoglycerides by alcoholysis of triglycerides in organic media. Reaction parameters such as choice of solvent, choice of alcohol, and alcohol concentration were studied. When 12.5 mM tripalmitin was used as substrate, methyl-tert-butyl ether was the best solvent for alcoholysis at water activity 0.11. Ethanol gave the highest yield (97%) at an optimal ethanol concentration of 200-300 mM. At higher alcohol concentrations, the enzyme activity was substantially lowered. The enzyme preparation showed high stability in repeated-batch reactions.
| Original language | English |
|---|---|
| Pages (from-to) | 1042-1047 |
| Number of pages | 6 |
| Journal | Enzyme and Microbial Technology |
| Volume | 16 |
| Issue number | 12 |
| DOIs | |
| Publication status | Published - 1994 Jan 1 |
Subject classification (UKÄ)
- Biocatalysis and Enzyme Technology
Free keywords
- 2-monoglycerides
- alcoholysis
- hydrolysis
- Lipase
- operational stability
- organic solvents
- water activity