Local growth factors are beneficial for the autonomic reinnervation of transplanted islets in rats

Martina Kvist Reimer, SP Mokshagundam, K Wyler, Frank Sundler, Bo Ahrén, JI Stagner

Research output: Contribution to journalArticlepeer-review

38 Citations (SciVal)

Abstract

Introduction: Transplanted islets, being avascular and denervated, receive blood vessels and nerves from the recipient. Reinnervation may account in part for the normalization of islet function in islet transplants. Whether reinnervation is possible to augment is not known. Aims and Methodology: To explore whether reinnervation of transplanted islets is augmented by local addition of growth factors to the graft, syngeneic islets were transplanted to the pancreas of streptozotocin-diabetic Lewis rats with or without pellets locally releasing nerve growth factor (NGF) and vascular endothelial growth factor (VEGF), alone or in combination. The pellets released growth factors for 14 days at a rate of 20 ng/day. After 7 weeks, pancreatic tissue was processed for immunofluorescence of insulin and the neural markers neuropeptide Y (NPY) and tyrosine hydroxylase (TH). Results: Islets were larger and more numerous after treatment with NGF (p = 0.024) and with NGF in combination with VEGF (p = 0.044). Similarly, immunostaining for TH and the C-terminal flanking peptide of NPY (C-PON) was more pronounced after treatment with NGF in combination with VEGF than in controls (both p < 0.05). Conclusion: Local growth factor treatment has a beneficial effect on autonomic reinnervation as well as islet integrity and survival of the graft after islet transplantation in rats.
Original languageEnglish
Pages (from-to)392-397
JournalPancreas
Volume26
Issue number4
Publication statusPublished - 2003

Bibliographical note

The information about affiliations in this record was updated in December 2015.
The record was previously connected to the following departments: Neuroendocrine Cell Biology (013212008), Medicine (Lund) (013230025)

Subject classification (UKÄ)

  • Cell and Molecular Biology
  • Other Clinical Medicine

Keywords

  • transplantation
  • rat
  • neuropeptides
  • nerve growth factor
  • graft
  • autonomic nerves
  • diabetes
  • vascular endothelial growth factor

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