Localization of hormone-sensitive lipase to rat Sertoli cells and its expression in developing and degenerating testes

Lena Stenson; Anna Maria Hoffmann; Hindrik Mulder; Frank Sundler; Cecilia Holm; Anders Bergh; Gudrun Fredrikson

doi:10.1016/0014-5793(94)01185-0

Localization of hormone-sensitive lipase to rat Sertoli cells and its expression in developing and degenerating testes

Lena Stenson, Anna Maria Hoffmann, Hindrik Mulder, Frank Sundler, Cecilia Holm, Anders Bergh, Gudrun Fredrikson

Research output: Contribution to journal › Article › peer-review

Abstract

Using in situ hybridization, hormone-sensitive lipase was found to be expressed in a stage-dependent manner in Sertoli cells of rat testis. No expression was found in Leydig cells but expression in spermatids could not be excluded. These results suggest a role for hormone-sensitive lipase in the metabolism of lipid droplets in Sertoli cells, in contrast to its previously proposed function in steroid biosynthesis. The expression of testicular hormone-sensitive lipase mRNA and protein, both larger in size compared to other tissues, coincided with the onset of spermatogenesis and was dependent on scrotal localization of the testis, suggesting a temperature-dependent, pretranslational regulation of expression.

Original language	English
Pages (from-to)	125-130
Journal	FEBS Letters
Volume	355
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(94)01185-0
Publication status	Published - 1994

Bibliographical note

The information about affiliations in this record was updated in December 2015.
The record was previously connected to the following departments: Molecular Endocrinology (013212018), Neuroendocrine Cell Biology (013212008), Molecular Metabolism (013212001), Insulin Signal Transduction (013212014)

Subject classification (UKÄ)

Biological Sciences

Free keywords

Hormone sensitive lipase
Cholesterol ester hydrolase
Testis
Sertoli cell
In situ hybridization

Access to Document

10.1016/0014-5793(94)01185-0

Cite this

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title = "Localization of hormone-sensitive lipase to rat Sertoli cells and its expression in developing and degenerating testes",

abstract = "Using in situ hybridization, hormone-sensitive lipase was found to be expressed in a stage-dependent manner in Sertoli cells of rat testis. No expression was found in Leydig cells but expression in spermatids could not be excluded. These results suggest a role for hormone-sensitive lipase in the metabolism of lipid droplets in Sertoli cells, in contrast to its previously proposed function in steroid biosynthesis. The expression of testicular hormone-sensitive lipase mRNA and protein, both larger in size compared to other tissues, coincided with the onset of spermatogenesis and was dependent on scrotal localization of the testis, suggesting a temperature-dependent, pretranslational regulation of expression.",

keywords = "Hormone sensitive lipase, Cholesterol ester hydrolase, Testis, Sertoli cell, In situ hybridization",

author = "Lena Stenson and Hoffmann, {Anna Maria} and Hindrik Mulder and Frank Sundler and Cecilia Holm and Anders Bergh and Gudrun Fredrikson",

note = "The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Molecular Endocrinology (013212018), Neuroendocrine Cell Biology (013212008), Molecular Metabolism (013212001), Insulin Signal Transduction (013212014)",

year = "1994",

doi = "10.1016/0014-5793(94)01185-0",

language = "English",

volume = "355",

pages = "125--130",

journal = "FEBS Letters",

issn = "1873-3468",

publisher = "Wiley-Blackwell",

number = "2",

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TY - JOUR

T1 - Localization of hormone-sensitive lipase to rat Sertoli cells and its expression in developing and degenerating testes

AU - Stenson, Lena

AU - Hoffmann, Anna Maria

AU - Mulder, Hindrik

AU - Sundler, Frank

AU - Holm, Cecilia

AU - Bergh, Anders

AU - Fredrikson, Gudrun

N1 - The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Molecular Endocrinology (013212018), Neuroendocrine Cell Biology (013212008), Molecular Metabolism (013212001), Insulin Signal Transduction (013212014)

PY - 1994

Y1 - 1994

N2 - Using in situ hybridization, hormone-sensitive lipase was found to be expressed in a stage-dependent manner in Sertoli cells of rat testis. No expression was found in Leydig cells but expression in spermatids could not be excluded. These results suggest a role for hormone-sensitive lipase in the metabolism of lipid droplets in Sertoli cells, in contrast to its previously proposed function in steroid biosynthesis. The expression of testicular hormone-sensitive lipase mRNA and protein, both larger in size compared to other tissues, coincided with the onset of spermatogenesis and was dependent on scrotal localization of the testis, suggesting a temperature-dependent, pretranslational regulation of expression.

AB - Using in situ hybridization, hormone-sensitive lipase was found to be expressed in a stage-dependent manner in Sertoli cells of rat testis. No expression was found in Leydig cells but expression in spermatids could not be excluded. These results suggest a role for hormone-sensitive lipase in the metabolism of lipid droplets in Sertoli cells, in contrast to its previously proposed function in steroid biosynthesis. The expression of testicular hormone-sensitive lipase mRNA and protein, both larger in size compared to other tissues, coincided with the onset of spermatogenesis and was dependent on scrotal localization of the testis, suggesting a temperature-dependent, pretranslational regulation of expression.

KW - Hormone sensitive lipase

KW - Cholesterol ester hydrolase

KW - Testis

KW - Sertoli cell

KW - In situ hybridization

U2 - 10.1016/0014-5793(94)01185-0

DO - 10.1016/0014-5793(94)01185-0

M3 - Article

SN - 1873-3468

VL - 355

SP - 125

EP - 130

JO - FEBS Letters

JF - FEBS Letters

IS - 2

ER -

Localization of hormone-sensitive lipase to rat Sertoli cells and its expression in developing and degenerating testes

Abstract

Bibliographical note

Subject classification (UKÄ)

Free keywords

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