Localization of human neutrophil interleukin-8 (CXCL-8) to organelle(s) distinct from the classical granules and secretory vesicles.

Sara Pellmé, Matthias Mörgelin, Hans Tapper, Ulf-Henrik Mellqvist, Claes Dahlgren, Anna Karlsson

Research output: Contribution to journalArticlepeer-review

35 Citations (SciVal)

Abstract

Mature human neutrophils contain small amounts of interleukin-8 [CXC chemokine ligand 8 (CXCL-8)], which upon proinflammatory activation, increases significantly. It has been suggested that the CXCL-8 content of resting human neutrophils is stored in the secretory vesicles. Here, we have used a fractionation technique, which allows isolation of these vesicles, and we find that CXCL-8 neither colocalizes with the secretory vesicles nor with markers of any of the classical neutrophil granules. To increase resolution in the system, we induced CXCL-8 production by lipopolysaccharide. After 8 h of stimulation, CXCL-8 was visualized within the cell using immunoelectron microscopy. The images revealed CXCL-8-containing stuctures resembling neutrophil granules, and these were distinct from all known neutrophil organelles, as shown by double immunostaining. Further, the CXCL-8 organelle was present in nonstimulated neutrophil cytoplasts, entities lacking all other known granules and secretory vesicles. Upon fractionation of the cytoplasts, CXCL-8 was found to partly cofractionate with calnexin, a marker for endoplasmic reticulum (ER). Thus, part of CXCL-8 may be localized to the ER or ER-like structures in the neutrophil.
Original languageEnglish
Pages (from-to)564-573
JournalJournal of Leukocyte Biology
Volume79
DOIs
Publication statusPublished - 2006

Subject classification (UKÄ)

  • Cell and Molecular Biology

Keywords

  • cell activation
  • subcellular organization
  • chemokines
  • inflammation

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