Macromolecular changes and nano-structural arrangements in gliadin and glutenin films upon chemical modification: Relation to functionality.

Faiza Rasheed, William R Newson, Tomás Plivelic, Ramune Kuktaite, Mikael S Hedenqvist, Mikael Gällstedt, Eva Johansson

Research output: Contribution to journalArticlepeer-review

22 Citations (SciVal)

Abstract

Protein macromolecules adopted for biological and bio-based material functions are known to develop a structured protein network upon chemical modification. In this study, we aimed to evaluate the impact of chemical additives such as, NaOH, NH4OH and salicylic acid (SA), on the secondary and nano-structural transitions of wheat proteins. Further, the effect of chemically induced modifications in protein macromolecular structure was anticipated in relation to functional properties. The gliadin-NH4OH-SA film showed a supramolecular protein organization into hexagonal structures with 65Å lattice parameter, and other not previously observed structural entities having a characteristic distance of 50Å. Proteins in gliadin-NH4OH-SA films were highly polymerized, with increased amount of disulfide crosslinks and β-sheets, causing improved strength and stiffness. Glutenin and WG proteins with NH4OH-SA showed extensive aggregation and an increase in β-sheet content together with irreversible crosslinks. Irreversible crosslinks hindered a high order structure formation in glutenins, and this resulted in films with only moderately improved stiffness. Thus, formation of nano-hierarchical structures based on β-sheets and disulfide crosslinks are the major reasons of high strength and stiffness in wheat protein based films.
Original languageEnglish
Pages (from-to)151-159
JournalInternational Journal of Biological Macromolecules
Volume79
DOIs
Publication statusPublished - 2015

Subject classification (UKÄ)

  • Materials Chemistry

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