Abstract
Paramagnetic versions of the CLEAR supports were prepared by entrapment of magnetite particles during suspension polymerization of allylamine, trimethylolpropane ethoxylate (14/3 EO/OH) triacrylate, and trimethylolpropane trimethacrylate. Swelling studies in a broad range of solvents showed comparable swelling of magnetic beads and regular CLEAR beads. The application of the magnetic beads as the support in solid-phase synthesis of peptides (Leu-enkephalinamide, substance P, acyl carrier protein (65-74) amide, and amyloid beta-protein (34-42) amide) and small organic affinity ligands based on the s-triazine scaffold was demonstrated. Adequate stability of the beads and retention of their magnetic properties were observed throughout the synthesis steps.
Original language | English |
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Pages (from-to) | 129-141 |
Journal | International Journal of Peptide Research and Therapeutics |
Volume | 13 |
Issue number | 1-2 |
DOIs | |
Publication status | Published - 2007 |
Subject classification (UKÄ)
- Pharmacology and Toxicology
Free keywords
- suspension polymerization
- substance P
- s-triazine scaffold
- synthesis
- solid-phase
- resin
- magnetite
- magnetic support
- Leu-enkephalin
- CLEAR
- acyl carrier protein (65-74)
- amyloid beta-protein (34-42)