Mass spectrometric determination of selected microbial constituents using fused silica and chiral glass capillary gas chromatography

G. Odham; A. Tunlid; L. Larsson; P. A. Mårdh

doi:10.1007/BF02258874

Mass spectrometric determination of selected microbial constituents using fused silica and chiral glass capillary gas chromatography

G. Odham, A. Tunlid, L. Larsson, P. A. Mårdh

Lund University

Research output: Contribution to journal › Article › peer-review

Abstract

Applications of quantitative gas chromatography/mass spectrometry (selected ion monitoring) to clinical and ecological microbiology are described. The technique permits determination of selected microbial metabolites and can be used for the rapid diagnosis of, for example, tuberculosis by ion-focusing of specific branch-chain fatty acids. The technique is also used to determine diaminopimelic and muramic acids, and D- and L-amino acids separated by chiral gas chromatography, in the low picogram range. The latter method has the potential to detect and quantitate microbial populations.

Original language	English
Pages (from-to)	83-86
Journal	Chromatographia
Volume	16
Issue number	1
DOIs	https://doi.org/10.1007/BF02258874
Publication status	Published - 1982 Dec 1

Subject classification (UKÄ)

Analytical Chemistry

Free keywords

Chiral capillary GC
D- and L-alanine
Diaminopimelic acid
Fused silica capillary GC
Mass spectrometry
Muramic acid
Selected ion monitoring
Tuberculostearic acid

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/BF02258874

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title = "Mass spectrometric determination of selected microbial constituents using fused silica and chiral glass capillary gas chromatography",

abstract = "Applications of quantitative gas chromatography/mass spectrometry (selected ion monitoring) to clinical and ecological microbiology are described. The technique permits determination of selected microbial metabolites and can be used for the rapid diagnosis of, for example, tuberculosis by ion-focusing of specific branch-chain fatty acids. The technique is also used to determine diaminopimelic and muramic acids, and D- and L-amino acids separated by chiral gas chromatography, in the low picogram range. The latter method has the potential to detect and quantitate microbial populations.",

keywords = "Chiral capillary GC, D- and L-alanine, Diaminopimelic acid, Fused silica capillary GC, Mass spectrometry, Muramic acid, Selected ion monitoring, Tuberculostearic acid",

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year = "1982",

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doi = "10.1007/BF02258874",

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TY - JOUR

T1 - Mass spectrometric determination of selected microbial constituents using fused silica and chiral glass capillary gas chromatography

AU - Odham, G.

AU - Tunlid, A.

AU - Larsson, L.

AU - Mårdh, P. A.

PY - 1982/12/1

Y1 - 1982/12/1

N2 - Applications of quantitative gas chromatography/mass spectrometry (selected ion monitoring) to clinical and ecological microbiology are described. The technique permits determination of selected microbial metabolites and can be used for the rapid diagnosis of, for example, tuberculosis by ion-focusing of specific branch-chain fatty acids. The technique is also used to determine diaminopimelic and muramic acids, and D- and L-amino acids separated by chiral gas chromatography, in the low picogram range. The latter method has the potential to detect and quantitate microbial populations.

AB - Applications of quantitative gas chromatography/mass spectrometry (selected ion monitoring) to clinical and ecological microbiology are described. The technique permits determination of selected microbial metabolites and can be used for the rapid diagnosis of, for example, tuberculosis by ion-focusing of specific branch-chain fatty acids. The technique is also used to determine diaminopimelic and muramic acids, and D- and L-amino acids separated by chiral gas chromatography, in the low picogram range. The latter method has the potential to detect and quantitate microbial populations.

KW - Chiral capillary GC

KW - D- and L-alanine

KW - Diaminopimelic acid

KW - Fused silica capillary GC

KW - Mass spectrometry

KW - Muramic acid

KW - Selected ion monitoring

KW - Tuberculostearic acid

U2 - 10.1007/BF02258874

DO - 10.1007/BF02258874

M3 - Article

AN - SCOPUS:34250234162

SN - 0009-5893

VL - 16

SP - 83

EP - 86

JO - Chromatographia

JF - Chromatographia

IS - 1

ER -

Mass spectrometric determination of selected microbial constituents using fused silica and chiral glass capillary gas chromatography

Abstract

Subject classification (UKÄ)

Free keywords

UN SDGs

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