Meandering nanochannels for imaging of ultra-long DNA molecules

C. Freitag; J. Fritzsche; F. Persson; Kalim U. Mir; J. O. Tegenfeldt

Meandering nanochannels for imaging of ultra-long DNA molecules

C. Freitag, J. Fritzsche, F. Persson, Kalim U. Mir, J. O. Tegenfeldt

Research output: Chapter in Book/Report/Conference proceeding › Paper in conference proceeding › peer-review

Abstract

A new chip design for handling and studying chromosomal DNA is described. Folding the nanochannels enables us to image an entire yeast chromosome in a single field of view. Together with existing mapping techniques our new design opens up the possibility of directly analyzing entire chromosomes in a single field of view using fluorescence microscopy. This would enable, for example, identification of microorganisms on a single-cell basis and the complete physical mapping of the human genome, chromosome by chromosome.

Original language	English
Title of host publication	15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Pages	1758-1760
Number of pages	3
Volume	3
Publication status	Published - 2011 Dec 1
Event	15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States Duration: 2011 Oct 2 → 2011 Oct 6

Conference

Conference	15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Country/Territory	United States
City	Seattle, WA
Period	2011/10/02 → 2011/10/06

Subject classification (UKÄ)

Medical Genetics and Genomics (including Gene Therapy)
Cell Biology

Free keywords

Chromosomal DNA
DNA analysis
Genomics
Nanofluidics

Cite this

@inproceedings{16a122b654b54dc99acf6a3f6fc5bfd2,

title = "Meandering nanochannels for imaging of ultra-long DNA molecules",

abstract = "A new chip design for handling and studying chromosomal DNA is described. Folding the nanochannels enables us to image an entire yeast chromosome in a single field of view. Together with existing mapping techniques our new design opens up the possibility of directly analyzing entire chromosomes in a single field of view using fluorescence microscopy. This would enable, for example, identification of microorganisms on a single-cell basis and the complete physical mapping of the human genome, chromosome by chromosome.",

keywords = "Chromosomal DNA, DNA analysis, Genomics, Nanofluidics",

author = "C. Freitag and J. Fritzsche and F. Persson and Mir, {Kalim U.} and Tegenfeldt, {J. O.}",

year = "2011",

month = dec,

day = "1",

language = "English",

isbn = "9781618395955",

volume = "3",

pages = "1758--1760",

booktitle = "15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011",

note = "15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 ; Conference date: 02-10-2011 Through 06-10-2011",

}

Freitag, C, Fritzsche, J, Persson, F, Mir, KU & Tegenfeldt, JO 2011, Meandering nanochannels for imaging of ultra-long DNA molecules. in 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. vol. 3, pp. 1758-1760, 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011, Seattle, WA, United States, 2011/10/02.

TY - GEN

T1 - Meandering nanochannels for imaging of ultra-long DNA molecules

AU - Freitag, C.

AU - Fritzsche, J.

AU - Persson, F.

AU - Mir, Kalim U.

AU - Tegenfeldt, J. O.

PY - 2011/12/1

Y1 - 2011/12/1

N2 - A new chip design for handling and studying chromosomal DNA is described. Folding the nanochannels enables us to image an entire yeast chromosome in a single field of view. Together with existing mapping techniques our new design opens up the possibility of directly analyzing entire chromosomes in a single field of view using fluorescence microscopy. This would enable, for example, identification of microorganisms on a single-cell basis and the complete physical mapping of the human genome, chromosome by chromosome.

AB - A new chip design for handling and studying chromosomal DNA is described. Folding the nanochannels enables us to image an entire yeast chromosome in a single field of view. Together with existing mapping techniques our new design opens up the possibility of directly analyzing entire chromosomes in a single field of view using fluorescence microscopy. This would enable, for example, identification of microorganisms on a single-cell basis and the complete physical mapping of the human genome, chromosome by chromosome.

KW - Chromosomal DNA

KW - DNA analysis

KW - Genomics

KW - Nanofluidics

UR - http://www.scopus.com/inward/record.url?scp=84883809915&partnerID=8YFLogxK

M3 - Paper in conference proceeding

AN - SCOPUS:84883809915

SN - 9781618395955

VL - 3

SP - 1758

EP - 1760

BT - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011

T2 - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011

Y2 - 2 October 2011 through 6 October 2011

ER -

Meandering nanochannels for imaging of ultra-long DNA molecules

Abstract

Conference

Subject classification (UKÄ)

Free keywords

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