@inbook{292e7844c3054233b26383f04251ec8d,
title = "Measurement of Antibody Binding Affinity on Bacterial Surfaces Using Flow Cytometry",
abstract = "Antibody binding to bacterial surfaces plays a crucial role in immunity, and a key characteristic of this protein-protein interaction is the binding affinity. Determining the affinity of an antibody binding to its antigen is the first step in predicting the function in a physiological environment where other competing protein interactions may be present. Antibody-antigen affinity is often evaluated with isolated proteins. It is informative to also be able to assess antibody binding to a bacterial surface where many antigens might be present, including multiple copies of the specific antigen the antibody recognizes, and in a context where the antigen might be in a more natural conformation. In this chapter, we present a flow cytometry-based assay to measure and calculate the cell surface binding affinity or avidity of any mono- or polyclonal antibody solution.",
keywords = "Flow Cytometry, Antibody Affinity, Antigens, Antibodies",
author = "{Kumra Ahnlide}, Vibha and Pontus Nordenfelt",
note = "{\textcopyright} 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2023",
doi = "10.1007/978-1-0716-3243-7_17",
language = "English",
isbn = "978-1-0716-3242-0",
series = "Methods in molecular biology (Clifton, N.J.)",
publisher = "Springer",
pages = "251--259",
editor = "Pontus Nordenfelt and Mattias Collin",
booktitle = "Bacterial Pathogenesis",
edition = "2",
}