Mechanism of metabolite transfer in coupled two-enzyme reactions involving aldolase

Henrik Pettersson, Gösta Pettersson

Research output: Contribution to journalArticlepeer-review


Transient-state kinetic experiments and analyses have been performed to examine the validity of hitherto unchallenged evidence proposed to be indicative of a channelled transfer of triose phosphates from aldolase to glyceraldehyde-3-phosphate dehydrogenase and glycerol-3-phosphate dehydrogenase. The results lend no support to such proposals, but show that the kinetic behaviour of the examined aldolase-dehydrogenase reactions is fully consistent with a free-diffusion mechanism of metabolite transfer.
Original languageEnglish
Pages (from-to)371-376
JournalEuropean Journal of Biochemistry
Issue number2
Publication statusPublished - 1999

Subject classification (UKÄ)

  • Biological Sciences

Free keywords

  • aldolase
  • dehydrogenase
  • channelling
  • glycolysis


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