Mechanism of metabolite transfer in coupled two-enzyme reactions involving aldolase

Henrik Pettersson, Gösta Pettersson

Research output: Contribution to journalArticlepeer-review

Abstract

Transient-state kinetic experiments and analyses have been performed to examine the validity of hitherto unchallenged evidence proposed to be indicative of a channelled transfer of triose phosphates from aldolase to glyceraldehyde-3-phosphate dehydrogenase and glycerol-3-phosphate dehydrogenase. The results lend no support to such proposals, but show that the kinetic behaviour of the examined aldolase-dehydrogenase reactions is fully consistent with a free-diffusion mechanism of metabolite transfer.
Original languageEnglish
Pages (from-to)371-376
JournalEuropean Journal of Biochemistry
Volume262
Issue number2
DOIs
Publication statusPublished - 1999

Subject classification (UKÄ)

  • Biological Sciences

Keywords

  • aldolase
  • dehydrogenase
  • channelling
  • glycolysis

Fingerprint

Dive into the research topics of 'Mechanism of metabolite transfer in coupled two-enzyme reactions involving aldolase'. Together they form a unique fingerprint.

Cite this