Abstract
Transient-state kinetic experiments and analyses have been performed to examine the validity of hitherto unchallenged evidence proposed to be indicative of a channelled transfer of triose phosphates from aldolase to glyceraldehyde-3-phosphate dehydrogenase and glycerol-3-phosphate dehydrogenase. The results lend no support to such proposals, but show that the kinetic behaviour of the examined aldolase-dehydrogenase reactions is fully consistent with a free-diffusion mechanism of metabolite transfer.
Original language | English |
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Pages (from-to) | 371-376 |
Journal | European Journal of Biochemistry |
Volume | 262 |
Issue number | 2 |
DOIs | |
Publication status | Published - 1999 |
Subject classification (UKÄ)
- Biological Sciences
Keywords
- aldolase
- dehydrogenase
- channelling
- glycolysis