Abstract
Steady-state and transient-state kinetic experiments have been performed to test the proposal that there is a direct (channelled) transfer of NADH from one dehydrogenase to another if the two enzymes exhibit distinct chiral coenzyme specificity (A-side vs. B-side). The results lend no support to this proposal, but are fully consistent with a free-diffusion mechanism of NADH transfer irrespective of the chiral specificity of the enzymes.
Original language | English |
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Pages (from-to) | 149-156 |
Journal | BBA - Protein Structure and Molecular Enzymology |
Volume | 1385 |
Issue number | 1 |
DOIs | |
Publication status | Published - 1998 |
Subject classification (UKÄ)
- Basic Medicine
Keywords
- Dehydrogenase
- Channelling
- Enzyme kinetics