Abstract
In this study, the signal cascade transducing carbachol stimulation into c-fos expression in SH-SY5Y neuroblastoma cells was investigated. 1,2-Diacylglycerol formation and c-fos expression were mediated via stimulation of muscarinic M1 receptors and the first 5 min of receptor stimulation were critical for these events. Application of 1,2-dioctanoylglycerol induced c-fos expression and this, as well as carbachol-stimulated c-fos expression, was inhibited by protein kinase C inhibitors. Increasing the intracellular Ca2+ concentration had only small effects on c-fos expression. There was a dependency on extracellular Ca2+ for maximal c-fos expression and 1,2-diacylglycerol formation. The carbachol-stimulated c-fos expression was potentiated by application of the protein phosphatase inhibitor okadaic acid. These results demonstrate the importance of 1,2-diacylglycerol formation for muscarinic receptor-stimulated, protein kinase C-mediated c-fos expression in the SH-SY5Y cells and that this cascade is counteracted by an okadaic acid-sensitive protein phosphatase.
| Original language | English |
|---|---|
| Pages (from-to) | 19-28 |
| Journal | European Journal of Pharmacology |
| Volume | 268 |
| Issue number | 1 |
| Publication status | Published - 1994 |
Bibliographical note
The information about affiliations in this record was updated in December 2015.The record was previously connected to the following departments: Tumour Cell Biology (013017530), Clinical Chemistry, Malmö (013016000), Division of Clinical Chemistry and Pharmacology (013250300)
Subject classification (UKÄ)
- Pharmacology and Toxicology