Metabolomic evaluation of pulsed electric field-induced stress on potato tissue

Federico Gomez; Petr Dejmek; Krister Lundgren; Allan Rasmusson; Antonio Vicente; Thomas Moritz

doi:10.1007/s00425-009-0950-2

Metabolomic evaluation of pulsed electric field-induced stress on potato tissue

Federico Gomez, Petr Dejmek, Krister Lundgren, Allan Rasmusson, Antonio Vicente, Thomas Moritz

Plant Biology

Research output: Contribution to journal › Article › peer-review

Abstract

Metabolite profiling was used to characterize stress responses of potato tissue subjected to reversible electroporation, providing insights on how potato tissue responds to a physical stimulus such as pulsed electric fields (PEF), which is an artificial stress. Wounded potato tissue was subjected to field strengths ranging from 200 to 400 V/cm, with a single rectangular pulse of 1 ms. Electroporation was demonstrated by propidium iodide staining of the cell nucleae. Metabolic profiling of data obtained through GC/TOF-MS and UPLC/TOF-MS complemented with orthogonal projections to latent structures clustering analysis showed that 24 h after the application of PEF, potato metabolism shows PEF-specific responses characterized by the changes in the hexose pool that may involve starch and ascorbic acid degradation.

Original language	English
Pages (from-to)	469-479
Journal	Planta
Volume	230
Issue number	3
DOIs	https://doi.org/10.1007/s00425-009-0950-2
Publication status	Published - 2009

Subject classification (UKÄ)

Other Engineering and Technologies
Biological Sciences

Free keywords

Electroporation
Cell membrane recovery
Metabolic analysis
multivariate data analysis
OPLS
Wounding
Stress response

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10.1007/s00425-009-0950-2

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title = "Metabolomic evaluation of pulsed electric field-induced stress on potato tissue",

abstract = "Metabolite profiling was used to characterize stress responses of potato tissue subjected to reversible electroporation, providing insights on how potato tissue responds to a physical stimulus such as pulsed electric fields (PEF), which is an artificial stress. Wounded potato tissue was subjected to field strengths ranging from 200 to 400 V/cm, with a single rectangular pulse of 1 ms. Electroporation was demonstrated by propidium iodide staining of the cell nucleae. Metabolic profiling of data obtained through GC/TOF-MS and UPLC/TOF-MS complemented with orthogonal projections to latent structures clustering analysis showed that 24 h after the application of PEF, potato metabolism shows PEF-specific responses characterized by the changes in the hexose pool that may involve starch and ascorbic acid degradation.",

keywords = "Electroporation, Cell membrane recovery, Metabolic analysis, multivariate data analysis, OPLS, Wounding, Stress response",

author = "Federico Gomez and Petr Dejmek and Krister Lundgren and Allan Rasmusson and Antonio Vicente and Thomas Moritz",

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doi = "10.1007/s00425-009-0950-2",

language = "English",

volume = "230",

pages = "469--479",

journal = "Planta",

issn = "0032-0935",

publisher = "Springer Science and Business Media B.V.",

number = "3",

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TY - JOUR

T1 - Metabolomic evaluation of pulsed electric field-induced stress on potato tissue

AU - Gomez, Federico

AU - Dejmek, Petr

AU - Lundgren, Krister

AU - Rasmusson, Allan

AU - Vicente, Antonio

AU - Moritz, Thomas

PY - 2009

Y1 - 2009

N2 - Metabolite profiling was used to characterize stress responses of potato tissue subjected to reversible electroporation, providing insights on how potato tissue responds to a physical stimulus such as pulsed electric fields (PEF), which is an artificial stress. Wounded potato tissue was subjected to field strengths ranging from 200 to 400 V/cm, with a single rectangular pulse of 1 ms. Electroporation was demonstrated by propidium iodide staining of the cell nucleae. Metabolic profiling of data obtained through GC/TOF-MS and UPLC/TOF-MS complemented with orthogonal projections to latent structures clustering analysis showed that 24 h after the application of PEF, potato metabolism shows PEF-specific responses characterized by the changes in the hexose pool that may involve starch and ascorbic acid degradation.

AB - Metabolite profiling was used to characterize stress responses of potato tissue subjected to reversible electroporation, providing insights on how potato tissue responds to a physical stimulus such as pulsed electric fields (PEF), which is an artificial stress. Wounded potato tissue was subjected to field strengths ranging from 200 to 400 V/cm, with a single rectangular pulse of 1 ms. Electroporation was demonstrated by propidium iodide staining of the cell nucleae. Metabolic profiling of data obtained through GC/TOF-MS and UPLC/TOF-MS complemented with orthogonal projections to latent structures clustering analysis showed that 24 h after the application of PEF, potato metabolism shows PEF-specific responses characterized by the changes in the hexose pool that may involve starch and ascorbic acid degradation.

KW - Electroporation

KW - Cell membrane recovery

KW - Metabolic analysis

KW - multivariate data analysis

KW - OPLS

KW - Wounding

KW - Stress response

U2 - 10.1007/s00425-009-0950-2

DO - 10.1007/s00425-009-0950-2

M3 - Article

C2 - 19495789

SN - 0032-0935

VL - 230

SP - 469

EP - 479

JO - Planta

JF - Planta

IS - 3

ER -

Metabolomic evaluation of pulsed electric field-induced stress on potato tissue

Abstract

Subject classification (UKÄ)

Free keywords

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