BACKGROUND: The study was carried out to evaluate the expression of major histocompatibility complex (MHC) molecules in retinal transplants with different tissue integrity.
METHODS: Twelve adult rabbits received an allogeneic subretinal neuroretinal transplant, in the form of either fragmented embryonic cells or a complete full-thickness embryonic retina. A controlled transvitreal approach was used for both transplantation types. The grafts were examined histologically after 31 or 49 days with hematoxylin and eosin staining and immunohistochemical analysis of MHC class I and class II expression.
RESULTS: All five fragment transplants developed into rosettes. Two of them displayed MHC class I-labeled cells, and four MHC class II-labeled cells. The cells were concentrated on the scleral side of the graft, and there was also a marked increase of labeled cells in the choroid. MHC labeling was often associated with defects in the retinal pigment epithelium. Six of the seven full-thickness grafts displayed a laminated morphology with well-developed retinal layers. The seventh consisted of rosettes. None of these grafts displayed MHC class I- or class II-labeled cells.
CONCLUSIONS: The findings suggest that host immune response against fragmented and intact neuroretinal grafts is different, indicating tissue integrity as one factor affecting graft-host immune interactions. The absence of immune response in full-thickness grafts is encouraging and important in the struggle to find therapies for retinal degenerative disease.
|Number of pages||10|
|Journal||Graefe's Archive for Clinical and Experimental Ophthalmology|
|Publication status||Published - 2000 Jul|
Subject classification (UKÄ)
- Fetal Tissue Transplantation
- Fluorescent Antibody Technique, Indirect
- Graft Rejection
- Histocompatibility Antigens Class I
- Histocompatibility Antigens Class II
- Microscopy, Fluorescence
- Pigment Epithelium of Eye
- Transplantation, Homologous
- Comparative Study
- Journal Article
- Research Support, Non-U.S. Gov't