Microfluidic-Derived Detection of Protein-Facilitated Copper Flux Across Lipid Membranes

Kamil Górecki, Jesper S. Hansen, Ping Li, Niloofar Nayeri, Karin Lindkvist-Petersson, Pontus Gourdon

Research output: Contribution to journalArticlepeer-review


Measurement of protein-facilitated copper flux across biological membranes is a considerable challenge. Here, we demonstrate a straightforward microfluidic-derived approach for visualization and measurement of membranous Cu flux. Giant unilamellar vesicles, reconstituted with the membrane protein of interest, are prepared, surface-immobilized, and assessed using a novel quencher-sensor reporter system for detection of copper. With the aid of a syringe pump, the external buffer is exchanged, enabling consistent and precise exchange of solutes, without causing vesicle rupture or uneven local metal concentrations brought about by rapid mixing. This approach bypasses common issues encountered when studying heavy metal-ion flux, thereby providing a new platform for in vitro studies of metal homeostasis aspects that are critical for all cells, health, and disease.

Original languageEnglish
Pages (from-to)11831-11837
Number of pages7
JournalAnalytical Chemistry
Issue number34
Publication statusPublished - 2022 Aug 30

Subject classification (UKÄ)

  • Biophysics
  • Medical Biotechnology


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