Microfluidic separation of parasites and parasite-infected cells from blood for the diagnosis of leishmaniasis

C. Regnault; K. Punyani; S. Holm; J. P. Beech; Marcia C. de Oliveira Otto; Kevan C Herold; M. Herbig; J. Guck; J. O. Tegenfeldt; Michael P Barrett

Microfluidic separation of parasites and parasite-infected cells from blood for the diagnosis of leishmaniasis

C. Regnault, K. Punyani, S. Holm, J. P. Beech, Marcia C. de Oliveira Otto, Kevan C Herold, M. Herbig, J. Guck, J. O. Tegenfeldt, Michael P Barrett

Research output: Chapter in Book/Report/Conference proceeding › Paper in conference proceeding › peer-review

Abstract

Microfluidic techniques were applied to the separation of parasite and parasite-infected cells from blood to facilitate the detection of leishmaniasis, a disease representing a high burden in the developing world and for which new diagnostic tools are urgently needed. Leishmania mexicana promastigotes were successfully separated from red blood cells in a deterministic lateral displacement device. The mechanical properties of macrophages infected with L. mexicana were investigated using real-time deformability cytometry. In the early stage, we find that macrophages deform less than the control. The trend is reversed four days post infection while we see a continuous increase in cell size after parasitization.

Original language	English
Title of host publication	20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
Publisher	Chemical and Biological Microsystems Society
Pages	244-245
Number of pages	2
ISBN (Electronic)	9780979806490
Publication status	Published - 2016
Event	20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, Ireland Duration: 2016 Oct 9 → 2016 Oct 13

Conference

Conference	20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
Country/Territory	Ireland
City	Dublin
Period	2016/10/09 → 2016/10/13

Subject classification (UKÄ)

Medical Laboratory and Measurements Technologies
Other Physics Topics

Free keywords

Deformability
Diagnosis
Leishmaniasis
Sorting

Cite this

Regnault, C., Punyani, K., Holm, S., Beech, J. P., Otto, M. C. D. O., Herold, K. C., Herbig, M., Guck, J., Tegenfeldt, J. O., & Barrett, M. P. (2016). Microfluidic separation of parasites and parasite-infected cells from blood for the diagnosis of leishmaniasis. In 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 (pp. 244-245). Chemical and Biological Microsystems Society.

@inproceedings{1baebc207549416ca8933ff713d8aca9,

title = "Microfluidic separation of parasites and parasite-infected cells from blood for the diagnosis of leishmaniasis",

abstract = "Microfluidic techniques were applied to the separation of parasite and parasite-infected cells from blood to facilitate the detection of leishmaniasis, a disease representing a high burden in the developing world and for which new diagnostic tools are urgently needed. Leishmania mexicana promastigotes were successfully separated from red blood cells in a deterministic lateral displacement device. The mechanical properties of macrophages infected with L. mexicana were investigated using real-time deformability cytometry. In the early stage, we find that macrophages deform less than the control. The trend is reversed four days post infection while we see a continuous increase in cell size after parasitization.",

keywords = "Deformability, Diagnosis, Leishmaniasis, Sorting",

author = "C. Regnault and K. Punyani and S. Holm and Beech, {J. P.} and Otto, {Marcia C. de Oliveira} and Herold, {Kevan C} and M. Herbig and J. Guck and Tegenfeldt, {J. O.} and Barrett, {Michael P}",

year = "2016",

language = "English",

pages = "244--245",

booktitle = "20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016",

publisher = "Chemical and Biological Microsystems Society",

note = "20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 ; Conference date: 09-10-2016 Through 13-10-2016",

}

Regnault, C, Punyani, K, Holm, S, Beech, JP, Otto, MCDO, Herold, KC, Herbig, M, Guck, J, Tegenfeldt, JO & Barrett, MP 2016, Microfluidic separation of parasites and parasite-infected cells from blood for the diagnosis of leishmaniasis. in 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society, pp. 244-245, 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016, Dublin, Ireland, 2016/10/09.

Microfluidic separation of parasites and parasite-infected cells from blood for the diagnosis of leishmaniasis. / Regnault, C.; Punyani, K.; Holm, S. et al.
20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society, 2016. p. 244-245.

Research output: Chapter in Book/Report/Conference proceeding › Paper in conference proceeding › peer-review

TY - GEN

T1 - Microfluidic separation of parasites and parasite-infected cells from blood for the diagnosis of leishmaniasis

AU - Regnault, C.

AU - Punyani, K.

AU - Holm, S.

AU - Beech, J. P.

AU - Otto, Marcia C. de Oliveira

AU - Herold, Kevan C

AU - Herbig, M.

AU - Guck, J.

AU - Tegenfeldt, J. O.

AU - Barrett, Michael P

PY - 2016

Y1 - 2016

N2 - Microfluidic techniques were applied to the separation of parasite and parasite-infected cells from blood to facilitate the detection of leishmaniasis, a disease representing a high burden in the developing world and for which new diagnostic tools are urgently needed. Leishmania mexicana promastigotes were successfully separated from red blood cells in a deterministic lateral displacement device. The mechanical properties of macrophages infected with L. mexicana were investigated using real-time deformability cytometry. In the early stage, we find that macrophages deform less than the control. The trend is reversed four days post infection while we see a continuous increase in cell size after parasitization.

AB - Microfluidic techniques were applied to the separation of parasite and parasite-infected cells from blood to facilitate the detection of leishmaniasis, a disease representing a high burden in the developing world and for which new diagnostic tools are urgently needed. Leishmania mexicana promastigotes were successfully separated from red blood cells in a deterministic lateral displacement device. The mechanical properties of macrophages infected with L. mexicana were investigated using real-time deformability cytometry. In the early stage, we find that macrophages deform less than the control. The trend is reversed four days post infection while we see a continuous increase in cell size after parasitization.

KW - Deformability

KW - Diagnosis

KW - Leishmaniasis

KW - Sorting

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M3 - Paper in conference proceeding

AN - SCOPUS:85014166507

SP - 244

EP - 245

BT - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

PB - Chemical and Biological Microsystems Society

T2 - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

Y2 - 9 October 2016 through 13 October 2016

ER -

Microfluidic separation of parasites and parasite-infected cells from blood for the diagnosis of leishmaniasis

Abstract

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Subject classification (UKÄ)

Free keywords

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