MicroRNA-340-5p inhibits colon cancer cell migration via targeting of RhoA

Anwar Algaber, Amr Al-Haidari, Raed Madhi, Milladur Rahman, Ingvar Syk, Henrik Thorlacius

Research output: Contribution to journalArticlepeer-review

Abstract

Colon cancer is the third most common cancer and a significant cause of cancer-related deaths worldwide. Metastasis is the most insidious aspect of cancer progression. Convincing data suggest that microRNAs (miRs) play a key function in colon cancer biology. We examined the role of miR-340-5p in regulating RhoA expression as well as cell migration and invasion in colon cancer cells. Levels of miR-340-5p and RhoA mRNA varied inversely in serum-free and serum-grown HT-29 and AZ-97 colon cancer cells. It was found transfection with miR-340-5p not only decreased expression of RhoA mRNA and protein levels in HT-29 cells but also reduced colon cancer cell migration and invasion. Bioinformatics analysis predicted one putative binding sites at the 3'-UTR of RhoA mRNA. Targeting this binding site with a specific blocker reversed mimic miR-340-5p-induced inhibition of RhoA activation and colon cancer cell migration and invasion. These novel results suggest that miR-340-5p is an important regulator of colon cancer cell motility via targeting of RhoA and further experiments are warranted to evaluate the role of miR-340-5p in colon cancer metastasis.

Original languageEnglish
Pages (from-to)16934
JournalScientific Reports
Volume10
Issue number1
DOIs
Publication statusPublished - 2020 Oct 9

Subject classification (UKÄ)

  • Cell and Molecular Biology
  • Cancer and Oncology

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