Microwave-assisted rapid characterization of lipase selectivities.

Sapna Bradoo, Pooja Rathi, R K Saxena, Rani Gupta

Research output: Contribution to journalArticlepeer-review

53 Citations (SciVal)

Abstract

A rapid screening procedure for characterization of lipase selectivities using microwaves was developed. The rate of reaction of various commercial lipases (porcine pancreas, Mucor miehei, Candida rugosa, Pseudomonas cepacia) as well as lipases from laboratory isolates-Bacillus stearothermophilus and Burkholderia cepacia RGP-10 for triolein hydrolysis was 7- to 12-fold higher in a microwave oven as compared to that by pH stat. The esterification of sucrose/methanol and ascorbic acid with different fatty acids was also achieved within 30 s in a microwave using porcine pancreas, B. stearothermophilus SB-1 and B. cepacia RGP-10 lipases. The relative rates and selectivity of the lipases both for hydrolytic and synthesis reactions remains unaltered. However, the rate of reaction was dynamically enhanced when exposed to microwaves. Microwave-assisted enzyme catalysis can become an attractive procedure for rapid characterization of large number of enzyme samples and substrates, which otherwise is a cumbersome and time-consuming exercise.
Original languageEnglish
Pages (from-to)115-120
JournalJournal of Biochemical and Biophysical Methods
Volume51
Issue number2
DOIs
Publication statusPublished - 2002

Subject classification (UKÄ)

  • Industrial Biotechnology

Keywords

  • Microwaves
  • Lipase : metabolism
  • Lipase : chemistry
  • Hydrolysis
  • Hydrogen-Ion Concentration
  • Biochemistry : methods
  • Bacteria : enzymology
  • Ascorbic Acid : chemistry
  • Animal
  • Time Factors
  • Triglycerides : chemistry
  • Substrate Specificity
  • Sucrose : chemistry
  • Support
  • Non-U.S. Gov't
  • Swine

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