Mitochondrial respiration in human viable platelets-Methodology and influence of gender, age and storage.

Fredrik Sjövall; Johannes Ehinger; Sigurdur Marelsson; Saori Morota; Eleonor Åsander Frostner; Hiroyuki Uchino; Johan Lundgren; Einar Arnbjörnsson; Magnus Hansson; Vineta Fellman; Eskil Elmer

doi:10.1016/j.mito.2012.11.001

Mitochondrial respiration in human viable platelets-Methodology and influence of gender, age and storage.

Fredrik Sjövall, Johannes Ehinger, Sigurdur Marelsson, Saori Morota, Eleonor Åsander Frostner, Hiroyuki Uchino, Johan Lundgren, Einar Arnbjörnsson, Magnus Hansson, Vineta Fellman, Eskil Elmer

Research output: Contribution to journal › Article › peer-review

Abstract

Studying whole cell preparations with intact mitochondria and respiratory complexes has a clear benefit compared to isolated or disrupted mitochondria due to the dynamic interplay between mitochondria and other cellular compartments. Platelet mitochondria have a potential to serve as a source of human viable mitochondria when studying mitochondrial physiology and pathogenic mechanisms, as well as for the diagnostics of mitochondrial diseases. The objective of the present study was to perform a detailed evaluation of platelet mitochondrial respiration using high-resolution respirometry. Further, we aimed to explore the limits of sample size and the impact of storage as well as to establish a wide range of reference data from different pediatric and adult cohorts. Our results indicate that platelet mitochondria are well suited for ex-vivo analysis with the need for minute sample amounts and excellent reproducibility and stability.

Original language	English
Pages (from-to)	7-14
Journal	Mitochondrion
Volume	13
Issue number	1
DOIs	https://doi.org/10.1016/j.mito.2012.11.001
Publication status	Published - 2013

Subject classification (UKÄ)

Respiratory Medicine and Allergy
Cell and Molecular Biology
Cardiology and Cardiovascular Disease

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.mito.2012.11.001

http://www.ncbi.nlm.nih.gov/pubmed/23164798?dopt=Abstract

1 Doctoral Thesis (compilation)

Mitochondrial function in sepsis - Temporal evolvement of respiratory capacity in human blood cells
Sjövall, F., 2013, Department of Clinical Sciences, Lund University. 83 p.
Research output: Thesis › Doctoral Thesis (compilation)

File

1 Finished

MITOEAGLE: EU FP Horizon 2020 COST Action CA15203 MITOEAGLE
Elmer, E. (PI), Åsander Frostner, E. (Research engineer), Hansson, M. (Researcher), Sjövall, F. (Researcher), Piel, S. (Research student), Ehinger, J. (Research student), Karlsson, M. (Research student), Westerlund, E. (Research student), Tavecchio, M. (Researcher) & Gnaiger, E. (PI)
2016/09/12 → 2020/09/11
Project: Network

2 Participation in conference
1 Participation in workshop/ seminar/ course

Mitochondrial fitness mapping: Conference, Working Groups and MC meeting
Åsander Frostner, E. (Role not specified)
2017 Mar 21 → 2017 Mar 23
Activity: Participating in or organising an event › Participation in conference
A roadmap for MITOEAGLE and first steps
Åsander Frostner, E. (Speaker) & Westerlund, E. (Speaker)
2016 Nov 15 → 2016 Nov 17
Activity: Participating in or organising an event › Participation in workshop/ seminar/ course
Scandomit 2010
Åsander Frostner, E. (Participant)
2010 Jan 20 → 2016 Jan 22
Activity: Participating in or organising an event › Participation in conference

Cite this

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title = "Mitochondrial respiration in human viable platelets-Methodology and influence of gender, age and storage.",

abstract = "Studying whole cell preparations with intact mitochondria and respiratory complexes has a clear benefit compared to isolated or disrupted mitochondria due to the dynamic interplay between mitochondria and other cellular compartments. Platelet mitochondria have a potential to serve as a source of human viable mitochondria when studying mitochondrial physiology and pathogenic mechanisms, as well as for the diagnostics of mitochondrial diseases. The objective of the present study was to perform a detailed evaluation of platelet mitochondrial respiration using high-resolution respirometry. Further, we aimed to explore the limits of sample size and the impact of storage as well as to establish a wide range of reference data from different pediatric and adult cohorts. Our results indicate that platelet mitochondria are well suited for ex-vivo analysis with the need for minute sample amounts and excellent reproducibility and stability.",

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T1 - Mitochondrial respiration in human viable platelets-Methodology and influence of gender, age and storage.

AU - Sjövall, Fredrik

AU - Ehinger, Johannes

AU - Marelsson, Sigurdur

AU - Morota, Saori

AU - Åsander Frostner, Eleonor

AU - Uchino, Hiroyuki

AU - Lundgren, Johan

AU - Arnbjörnsson, Einar

AU - Hansson, Magnus

AU - Fellman, Vineta

AU - Elmer, Eskil

PY - 2013

Y1 - 2013

N2 - Studying whole cell preparations with intact mitochondria and respiratory complexes has a clear benefit compared to isolated or disrupted mitochondria due to the dynamic interplay between mitochondria and other cellular compartments. Platelet mitochondria have a potential to serve as a source of human viable mitochondria when studying mitochondrial physiology and pathogenic mechanisms, as well as for the diagnostics of mitochondrial diseases. The objective of the present study was to perform a detailed evaluation of platelet mitochondrial respiration using high-resolution respirometry. Further, we aimed to explore the limits of sample size and the impact of storage as well as to establish a wide range of reference data from different pediatric and adult cohorts. Our results indicate that platelet mitochondria are well suited for ex-vivo analysis with the need for minute sample amounts and excellent reproducibility and stability.

AB - Studying whole cell preparations with intact mitochondria and respiratory complexes has a clear benefit compared to isolated or disrupted mitochondria due to the dynamic interplay between mitochondria and other cellular compartments. Platelet mitochondria have a potential to serve as a source of human viable mitochondria when studying mitochondrial physiology and pathogenic mechanisms, as well as for the diagnostics of mitochondrial diseases. The objective of the present study was to perform a detailed evaluation of platelet mitochondrial respiration using high-resolution respirometry. Further, we aimed to explore the limits of sample size and the impact of storage as well as to establish a wide range of reference data from different pediatric and adult cohorts. Our results indicate that platelet mitochondria are well suited for ex-vivo analysis with the need for minute sample amounts and excellent reproducibility and stability.

U2 - 10.1016/j.mito.2012.11.001

DO - 10.1016/j.mito.2012.11.001

M3 - Article

C2 - 23164798

SN - 1567-7249

VL - 13

SP - 7

EP - 14

JO - Mitochondrion

JF - Mitochondrion

IS - 1

ER -

Mitochondrial respiration in human viable platelets-Methodology and influence of gender, age and storage.

Abstract

Subject classification (UKÄ)

UN SDGs

Access to Document

Fingerprint

Research output

Mitochondrial function in sepsis - Temporal evolvement of respiratory capacity in human blood cells

Projects

MITOEAGLE: EU FP Horizon 2020 COST Action CA15203 MITOEAGLE

Activities

Mitochondrial fitness mapping: Conference, Working Groups and MC meeting

A roadmap for MITOEAGLE and first steps

Scandomit 2010

Cite this