Modeling ebola virus genome replication and transcription with minigenome systems

Tessa Cressey, Kristina Brauburger, Elke Mühlberger

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

Abstract

In this chapter, we describe the minigenome system for Ebola virus (EBOV), which reconstitutes EBOV polymerase activity in cells and can be used to model viral genome replication and transcription. This protocol comprises all steps including cell culture, plasmid preparation, transfection, and luciferase reporter assay readout.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
EditorsThomas Hoenen, Allison Groseth
PublisherHumana Press
Pages79-92
Number of pages14
Volume1628
DOIs
Publication statusPublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1628
ISSN (Print)10643745

Subject classification (UKÄ)

  • Cell Biology

Free keywords

  • Ebola virus
  • Filoviruses
  • Firefly luciferase assay
  • Minigenome
  • Replication
  • Replication promoter
  • Reporter gene
  • Transcription
  • Zaire ebolavirus
  • β-galactosidase enzyme assay

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