Abstract
In this chapter, we describe the minigenome system for Ebola virus (EBOV), which reconstitutes EBOV polymerase activity in cells and can be used to model viral genome replication and transcription. This protocol comprises all steps including cell culture, plasmid preparation, transfection, and luciferase reporter assay readout.
| Original language | English |
|---|---|
| Title of host publication | Methods in Molecular Biology |
| Editors/authors | Thomas Hoenen, Allison Groseth |
| Publisher | Humana Press |
| Pages | 79-92 |
| Number of pages | 14 |
| Volume | 1628 |
| DOIs | |
| Publication status | Published - 2017 |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Volume | 1628 |
| ISSN (Print) | 10643745 |
UN SDGs
This output contributes to the following UN Sustainable Development Goals (SDGs)
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SDG 3 Good Health and Well-being
Subject classification (UKÄ)
- Cell Biology
Free keywords
- Ebola virus
- Filoviruses
- Firefly luciferase assay
- Minigenome
- Replication
- Replication promoter
- Reporter gene
- Transcription
- Zaire ebolavirus
- β-galactosidase enzyme assay
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