Molecular Cloning and Biochemical Characterization of Iron Superoxide Dismutase from Leishmania braziliensis

Camila C.B. Brito; Fernando V. Maluf; Gustavo M.A. de Lima; Rafael V.C. Guido; Marcelo S. Castilho

doi:10.1007/s12033-018-0095-2

Molecular Cloning and Biochemical Characterization of Iron Superoxide Dismutase from Leishmania braziliensis

Camila C.B. Brito, Fernando V. Maluf, Gustavo M.A. de Lima, Rafael V.C. Guido, Marcelo S. Castilho

MAX IV Laboratory

Research output: Contribution to journal › Article › peer-review

Abstract

Leishmaniasis is one of the most important neglected tropical diseases, with a broad spectrum of clinical manifestations. Among the clinical manifestations of the disease, cutaneous leishmaniasis, caused by species of Leishmania braziliensis, presents wide distribution in Brazil. In this work, we performed the cloning, expression, and purification of the enzyme superoxide dismutase of Leishmania braziliensis (LbSOD-B2) considered a promising target for the search of new compounds against leishmaniasis. In vitro assays based on pyrogallol oxidation showed that LbSOD-B2 is most active around pH 8 and hydrogen peroxide is a LbSOD-B2 inhibitor at low millimolar range (IC₅₀ = 1 mM).

Original language	English
Pages (from-to)	595-600
Journal	Molecular Biotechnology
Volume	60
Issue number	8
DOIs	https://doi.org/10.1007/s12033-018-0095-2
Publication status	Published - 2018

Subject classification (UKÄ)

Medical Biotechnology

Free keywords

Heterologous expression
Kinetic assay
Leishmania braziliensis
Superoxide dismutase

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s12033-018-0095-2

Cite this

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title = "Molecular Cloning and Biochemical Characterization of Iron Superoxide Dismutase from Leishmania braziliensis",

abstract = "Leishmaniasis is one of the most important neglected tropical diseases, with a broad spectrum of clinical manifestations. Among the clinical manifestations of the disease, cutaneous leishmaniasis, caused by species of Leishmania braziliensis, presents wide distribution in Brazil. In this work, we performed the cloning, expression, and purification of the enzyme superoxide dismutase of Leishmania braziliensis (LbSOD-B2) considered a promising target for the search of new compounds against leishmaniasis. In vitro assays based on pyrogallol oxidation showed that LbSOD-B2 is most active around pH 8 and hydrogen peroxide is a LbSOD-B2 inhibitor at low millimolar range (IC50 = 1 mM).",

keywords = "Heterologous expression, Kinetic assay, Leishmania braziliensis, Superoxide dismutase",

author = "Brito, {Camila C.B.} and Maluf, {Fernando V.} and {de Lima}, {Gustavo M.A.} and Guido, {Rafael V.C.} and Castilho, {Marcelo S.}",

year = "2018",

doi = "10.1007/s12033-018-0095-2",

language = "English",

volume = "60",

pages = "595--600",

journal = "Molecular Biotechnology",

issn = "1073-6085",

publisher = "Humana Press",

number = "8",

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TY - JOUR

T1 - Molecular Cloning and Biochemical Characterization of Iron Superoxide Dismutase from Leishmania braziliensis

AU - Brito, Camila C.B.

AU - Maluf, Fernando V.

AU - de Lima, Gustavo M.A.

AU - Guido, Rafael V.C.

AU - Castilho, Marcelo S.

PY - 2018

Y1 - 2018

N2 - Leishmaniasis is one of the most important neglected tropical diseases, with a broad spectrum of clinical manifestations. Among the clinical manifestations of the disease, cutaneous leishmaniasis, caused by species of Leishmania braziliensis, presents wide distribution in Brazil. In this work, we performed the cloning, expression, and purification of the enzyme superoxide dismutase of Leishmania braziliensis (LbSOD-B2) considered a promising target for the search of new compounds against leishmaniasis. In vitro assays based on pyrogallol oxidation showed that LbSOD-B2 is most active around pH 8 and hydrogen peroxide is a LbSOD-B2 inhibitor at low millimolar range (IC50 = 1 mM).

AB - Leishmaniasis is one of the most important neglected tropical diseases, with a broad spectrum of clinical manifestations. Among the clinical manifestations of the disease, cutaneous leishmaniasis, caused by species of Leishmania braziliensis, presents wide distribution in Brazil. In this work, we performed the cloning, expression, and purification of the enzyme superoxide dismutase of Leishmania braziliensis (LbSOD-B2) considered a promising target for the search of new compounds against leishmaniasis. In vitro assays based on pyrogallol oxidation showed that LbSOD-B2 is most active around pH 8 and hydrogen peroxide is a LbSOD-B2 inhibitor at low millimolar range (IC50 = 1 mM).

KW - Heterologous expression

KW - Kinetic assay

KW - Leishmania braziliensis

KW - Superoxide dismutase

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U2 - 10.1007/s12033-018-0095-2

DO - 10.1007/s12033-018-0095-2

M3 - Article

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AN - SCOPUS:85049114270

SN - 1073-6085

VL - 60

SP - 595

EP - 600

JO - Molecular Biotechnology

JF - Molecular Biotechnology

IS - 8

ER -

Molecular Cloning and Biochemical Characterization of Iron Superoxide Dismutase from Leishmania braziliensis

Abstract

Subject classification (UKÄ)

Free keywords

UN SDGs

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