Molecular profiling reveals distinct functional attributes of CD1d-restricted natural killer (NK) T cell subsets

Julia Rolf; Emma Berntman; Martin Stenström; Emma Smith; Robert Månsson; Hanna Stenstad; Tetsuya Yamagata; William Agace; Mikael Sigvardsson; Susanna Cardell

doi:10.1016/j.molimm.2007.12.022

Molecular profiling reveals distinct functional attributes of CD1d-restricted natural killer (NK) T cell subsets

Julia Rolf, Emma Berntman, Martin Stenström, Emma Smith, Robert Månsson, Hanna Stenstad, Tetsuya Yamagata, William Agace, Mikael Sigvardsson, Susanna Cardell

Research output: Contribution to journal › Article › peer-review

Abstract

CD1d-restricted natural killer T (NKT) cells can have multiple effects on an immune response, including the activation, regulation and attraction of innate immune cells, and modulation of adaptive immunity. Recent studies reveal that there are distinct subsets of NKT cells which selectively perform some of the functions attributed to CD1d-restricted cells, but the mechanisms underlying these functional differences have not been resolved. Our aim in this study was to identify novel NKT cell associated traits that would provide important insight into NKT cell activation and function. To this end, we have performed gene expression profiling of two separate subsets of NKT cells, analyzing genes differentially expressed in these cells compared to conventional CD4(+)NK1.1(-) T cells. We identify different sets of genes over expressed in each of the two NKT cell types, as well as genes that are common to the two CD1d-restricted NKT cell populations analyzed. A large number of these genes are highly relevant for NKT cell development, activation and function. Each NKT subtype displayed a unique set of chemokine receptors, integrins and molecules related to effector function, supporting the notion that distinct NKT cells can be selectively engaged and have diverse functions in different types of immune reactions.

Original language	English
Pages (from-to)	2607-2620
Journal	Molecular Immunology
Volume	45
Issue number	9
DOIs	https://doi.org/10.1016/j.molimm.2007.12.022
Publication status	Published - 2008

Bibliographical note

The information about affiliations in this record was updated in December 2015.
The record was previously connected to the following departments: Hematopoietic Stem Cell Laboratory (013022012), Immunology (013212020)

Subject classification (UKÄ)

Immunology in the medical area

Free keywords

gene expression
NKT cells
CD1d
transcription factors

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10.1016/j.molimm.2007.12.022

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title = "Molecular profiling reveals distinct functional attributes of CD1d-restricted natural killer (NK) T cell subsets",

abstract = "CD1d-restricted natural killer T (NKT) cells can have multiple effects on an immune response, including the activation, regulation and attraction of innate immune cells, and modulation of adaptive immunity. Recent studies reveal that there are distinct subsets of NKT cells which selectively perform some of the functions attributed to CD1d-restricted cells, but the mechanisms underlying these functional differences have not been resolved. Our aim in this study was to identify novel NKT cell associated traits that would provide important insight into NKT cell activation and function. To this end, we have performed gene expression profiling of two separate subsets of NKT cells, analyzing genes differentially expressed in these cells compared to conventional CD4(+)NK1.1(-) T cells. We identify different sets of genes over expressed in each of the two NKT cell types, as well as genes that are common to the two CD1d-restricted NKT cell populations analyzed. A large number of these genes are highly relevant for NKT cell development, activation and function. Each NKT subtype displayed a unique set of chemokine receptors, integrins and molecules related to effector function, supporting the notion that distinct NKT cells can be selectively engaged and have diverse functions in different types of immune reactions.",

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author = "Julia Rolf and Emma Berntman and Martin Stenstr{\"o}m and Emma Smith and Robert M{\aa}nsson and Hanna Stenstad and Tetsuya Yamagata and William Agace and Mikael Sigvardsson and Susanna Cardell",

note = "The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Hematopoietic Stem Cell Laboratory (013022012), Immunology (013212020)",

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AU - Berntman, Emma

AU - Stenström, Martin

AU - Smith, Emma

AU - Månsson, Robert

AU - Stenstad, Hanna

AU - Yamagata, Tetsuya

AU - Agace, William

AU - Sigvardsson, Mikael

AU - Cardell, Susanna

N1 - The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Hematopoietic Stem Cell Laboratory (013022012), Immunology (013212020)

PY - 2008

Y1 - 2008

N2 - CD1d-restricted natural killer T (NKT) cells can have multiple effects on an immune response, including the activation, regulation and attraction of innate immune cells, and modulation of adaptive immunity. Recent studies reveal that there are distinct subsets of NKT cells which selectively perform some of the functions attributed to CD1d-restricted cells, but the mechanisms underlying these functional differences have not been resolved. Our aim in this study was to identify novel NKT cell associated traits that would provide important insight into NKT cell activation and function. To this end, we have performed gene expression profiling of two separate subsets of NKT cells, analyzing genes differentially expressed in these cells compared to conventional CD4(+)NK1.1(-) T cells. We identify different sets of genes over expressed in each of the two NKT cell types, as well as genes that are common to the two CD1d-restricted NKT cell populations analyzed. A large number of these genes are highly relevant for NKT cell development, activation and function. Each NKT subtype displayed a unique set of chemokine receptors, integrins and molecules related to effector function, supporting the notion that distinct NKT cells can be selectively engaged and have diverse functions in different types of immune reactions.

AB - CD1d-restricted natural killer T (NKT) cells can have multiple effects on an immune response, including the activation, regulation and attraction of innate immune cells, and modulation of adaptive immunity. Recent studies reveal that there are distinct subsets of NKT cells which selectively perform some of the functions attributed to CD1d-restricted cells, but the mechanisms underlying these functional differences have not been resolved. Our aim in this study was to identify novel NKT cell associated traits that would provide important insight into NKT cell activation and function. To this end, we have performed gene expression profiling of two separate subsets of NKT cells, analyzing genes differentially expressed in these cells compared to conventional CD4(+)NK1.1(-) T cells. We identify different sets of genes over expressed in each of the two NKT cell types, as well as genes that are common to the two CD1d-restricted NKT cell populations analyzed. A large number of these genes are highly relevant for NKT cell development, activation and function. Each NKT subtype displayed a unique set of chemokine receptors, integrins and molecules related to effector function, supporting the notion that distinct NKT cells can be selectively engaged and have diverse functions in different types of immune reactions.

KW - gene expression

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DO - 10.1016/j.molimm.2007.12.022

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SN - 1872-9142

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Molecular profiling reveals distinct functional attributes of CD1d-restricted natural killer (NK) T cell subsets

Abstract

Bibliographical note

Subject classification (UKÄ)

Free keywords

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