Monitoring of glucose and glutamate using enzyme microstructures and scanning electrochemical microscopy.

Laura Muresan; Mihaela Nistor; Szilveszter Gáspár; Ionel Cătălin Popescu; Elisabeth Csöregi

doi:10.1016/j.bioelechem.2009.05.004

Monitoring of glucose and glutamate using enzyme microstructures and scanning electrochemical microscopy.

Laura Muresan, Mihaela Nistor, Szilveszter Gáspár, Ionel Cătălin Popescu, Elisabeth Csöregi

Centre for Analysis and Synthesis

Research output: Contribution to journal › Article › peer-review

Abstract

Glucose oxidase and glutamate oxidase lines, with typical width of 100 microm, were patterned on gold surfaces using a micro-dispensing system, by shooting 100 pl droplets of the corresponding enzyme solutions. The probe of a scanning electrochemical microscope (SECM) was then carefully positioned in the close proximity of the enzyme microstructure and poised to +600 mV vs. Ag/AgCl, KCl 0.1 M. The H(2)O(2), generated by the enzyme lines at different concentrations of glucose and glutamate in the surrounding solution, was sequentially monitored. Reproducible calibration curves for glucose and glutamate were obtained in one single experiment, proving that the combination of enzyme microstructures with SECM can provide a new way of achieving multianalyte detection.

Original language	English
Pages (from-to)	81-86
Journal	Bioelectrochemistry
Volume	76
DOIs	https://doi.org/10.1016/j.bioelechem.2009.05.004
Publication status	Published - 2009

Bibliographical note

The information about affiliations in this record was updated in December 2015.
The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)

Subject classification (UKÄ)

Analytical Chemistry

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10.1016/j.bioelechem.2009.05.004

http://www.ncbi.nlm.nih.gov/pubmed/19520620?dopt=Abstract

Cite this

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title = "Monitoring of glucose and glutamate using enzyme microstructures and scanning electrochemical microscopy.",

abstract = "Glucose oxidase and glutamate oxidase lines, with typical width of 100 microm, were patterned on gold surfaces using a micro-dispensing system, by shooting 100 pl droplets of the corresponding enzyme solutions. The probe of a scanning electrochemical microscope (SECM) was then carefully positioned in the close proximity of the enzyme microstructure and poised to +600 mV vs. Ag/AgCl, KCl 0.1 M. The H(2)O(2), generated by the enzyme lines at different concentrations of glucose and glutamate in the surrounding solution, was sequentially monitored. Reproducible calibration curves for glucose and glutamate were obtained in one single experiment, proving that the combination of enzyme microstructures with SECM can provide a new way of achieving multianalyte detection.",

author = "Laura Muresan and Mihaela Nistor and Szilveszter G{\'a}sp{\'a}r and Popescu, {Ionel C{\u a}t{\u a}lin} and Elisabeth Cs{\"o}regi",

note = "The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)",

year = "2009",

doi = "10.1016/j.bioelechem.2009.05.004",

language = "English",

volume = "76",

pages = "81--86",

journal = "Bioelectrochemistry",

issn = "1878-562X",

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TY - JOUR

T1 - Monitoring of glucose and glutamate using enzyme microstructures and scanning electrochemical microscopy.

AU - Muresan, Laura

AU - Nistor, Mihaela

AU - Gáspár, Szilveszter

AU - Popescu, Ionel Cătălin

AU - Csöregi, Elisabeth

N1 - The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)

PY - 2009

Y1 - 2009

N2 - Glucose oxidase and glutamate oxidase lines, with typical width of 100 microm, were patterned on gold surfaces using a micro-dispensing system, by shooting 100 pl droplets of the corresponding enzyme solutions. The probe of a scanning electrochemical microscope (SECM) was then carefully positioned in the close proximity of the enzyme microstructure and poised to +600 mV vs. Ag/AgCl, KCl 0.1 M. The H(2)O(2), generated by the enzyme lines at different concentrations of glucose and glutamate in the surrounding solution, was sequentially monitored. Reproducible calibration curves for glucose and glutamate were obtained in one single experiment, proving that the combination of enzyme microstructures with SECM can provide a new way of achieving multianalyte detection.

AB - Glucose oxidase and glutamate oxidase lines, with typical width of 100 microm, were patterned on gold surfaces using a micro-dispensing system, by shooting 100 pl droplets of the corresponding enzyme solutions. The probe of a scanning electrochemical microscope (SECM) was then carefully positioned in the close proximity of the enzyme microstructure and poised to +600 mV vs. Ag/AgCl, KCl 0.1 M. The H(2)O(2), generated by the enzyme lines at different concentrations of glucose and glutamate in the surrounding solution, was sequentially monitored. Reproducible calibration curves for glucose and glutamate were obtained in one single experiment, proving that the combination of enzyme microstructures with SECM can provide a new way of achieving multianalyte detection.

U2 - 10.1016/j.bioelechem.2009.05.004

DO - 10.1016/j.bioelechem.2009.05.004

M3 - Article

C2 - 19520620

SN - 1878-562X

VL - 76

SP - 81

EP - 86

JO - Bioelectrochemistry

JF - Bioelectrochemistry

ER -

Monitoring of glucose and glutamate using enzyme microstructures and scanning electrochemical microscopy.

Abstract

Bibliographical note

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