Morphology of living cells cultured on nanowire arrays with varying nanowire densities and diameters

Zhen Li, Henrik Persson, Karl Adolfsson, Stina Oredsson, Christelle N. Prinz

Research output: Contribution to journalArticlepeer-review

Abstract

Vertical nanowire arrays are increasingly investigated for their applications in steering cell behavior. The geometry of the array is an important parameter, which influences the morphology and adhesion of cells. Here, we investigate the effects of array geometry on the morphology of MCF7 cancer cells and MCF10A normal-like epithelial cells. Different gallium phosphide nanowire array-geometries were produced by varying the nanowire density and diameter. Our results show that the cell size is smaller on nanowires compared to flat gallium phosphide. The cell area decreases with increasing the nanowire density on the substrate. We observed an effect of the nanowire diameter on MCF10A cells, with a decreased cell area on 40 nm diameter nanowires, compared to 60 and 80 nm diameter nanowires in high-density arrays. The focal adhesion morphology depends on the extent to which cells are contacting the substrate. For low nanowire densities and diameters, cells are lying on the substrate and we observed large focal adhesions at the cell edges. In contrast, for high nanowire densities and diameters, cells are lying on top of the nanowires and we observed point-like focal adhesions distributed over the whole cell. Our results constitute a step towards the ability to fine-tune cell behavior on nanowire arrays.

Original languageEnglish
Pages (from-to)427-435
JournalScience China Life Sciences
Volume61
Issue number4
Early online date2018 Apr 2
DOIs
Publication statusPublished - 2018

Subject classification (UKÄ)

  • Nano Technology
  • Cell Biology
  • Other Physics Topics

Free keywords

  • aspect ratio
  • cell
  • density
  • diameter
  • morphology
  • nanowires

Fingerprint

Dive into the research topics of 'Morphology of living cells cultured on nanowire arrays with varying nanowire densities and diameters'. Together they form a unique fingerprint.

Cite this