Neuronal and glial differentiation within expanded glial cultures derived from the lateral and medial ganglionic eminences.

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Attached glial-like cell cultures were established from the lateral and medial ganglionic eminences (LGE and MGE) and from the neocortex (Cx) of E13.5 mouse embryos, and expanded over four to five passages under epidermal growth factor (EGF) stimulation. Following removal of EGF and serum, we analysed the generation of neurons and glial cells within the cultures. Significant numbers of βIII-tubulin-positive neurons were generated in both the LGE (about 7% of total cell numbers) and the MGE (around 2%). However, only few βIII-tubulin-positive cells with neuronal morphologies were detected in the differentiated Cx cultures. The newly formed neurons were to a large extent GABAergic, and many of the MGE-derived, but not the LGE-derived, cells expressed the MGE-marker NKX2.1. Most cells in all cultures still appeared astroglial-like, expressing glial fibrillary acidic protein (GFAP), but in addition, CNPase-positive cells with oligodendroglial morphologies were present in the MGE (0.68%), and, to a lesser extent (0.2%), in the LGE cultures. The present results demonstrate that cells of expanded glial cultures from both the LGE and MGE can give rise to significant and, to a certain extent, region-specific neuronal and glial cell types under differentiating conditions.
Original languageEnglish
Pages (from-to)1058-1063
JournalExperimental Neurology
Issue number2
Publication statusPublished - 2003

Bibliographical note

The information about affiliations in this record was updated in December 2015.
The record was previously connected to the following departments: Molecular Medicine and Gene Therapy (0131000135), Neurobiology (013212024), Neuronano Research Center (NRC) (013210020), Neurology, Lund (013027000)

Subject classification (UKÄ)

  • Neurology


  • Differentiation
  • Expansion
  • EGF
  • Astroglial cells
  • Telencephalon
  • Neurons
  • Oligodendrocytes


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