Neuronal death in nigral grafts in the absence of poly (ADP-ribose) polymerase activation

Gabriele Schierle, Oskar Hansson, E Ferrando-May, P Nicotera, Patrik Brundin, M Leist

Research output: Contribution to journalArticlepeer-review


The exact causes of the extensive cell death in nigral transplants are still unknown. Since poly-(ADP-ribose) polymerase (PARP) overactivation has been implicated in neuronal death, we examined the effects of PARP on the survival of nigral grafts by using donor tissue from PARP knock-out or wild-type mice. Eight hours after preparation of the nigral cell suspension, cell damage was quantified by measurement of lactate dehydrogenase release, DNA fragmentation and caspase activation. At this stage, PARP deletion had no protective effect. Moreover, neither the survival of transplanted dopaminergic neurons, nor the functional recovery of hemiparkinsonian graft recipients were improved by the absence of PARP. We conclude that cell death in embryonic nigral grafts is not affected by the absence of PARP activation.
Original languageEnglish
Pages (from-to)3347-3351
Issue number16
Publication statusPublished - 1999

Bibliographical note

The information about affiliations in this record was updated in December 2015.
The record was previously connected to the following departments: Psychiatry/Primary Care/Public Health (013240500), Department of Experimental Medical Science (013210000)

Subject classification (UKÄ)

  • Neurosciences


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