Complex biological and environmental samples require a high degree of selectivity in order to determine components therein. Sensitivity measures, such as analyte pre-concentration are often required for assessing composition quantitatively. Use of membranes and especially non-porous membranes for extraction purposes offers in many cases the desired measure of selectivity and sensitivity for trace analysis from such samples. Selected species can be either permanently charged, ionogenic or neutral, and range in size from protons to proteins. The result of the extractions is often excellent cleanup of the matrix and a decrease in detection limits. Another salient feature with these systems is that they offer an extremely low consumption of potentially hazardous, organic solvents. The work in this thesis has focused on novel approaches within the field of non-porous membrane extraction, including system integration and automation, miniaturisation and extensions of technical scope and applicability. Integration has been designed to suit a number of important techniques, such as normal-phase and reversed-phase liquid chromatography (LC), capillary-LC, gas chromatography (GC), capillary electrophoresis (CE) and continuous-flow systems (FIA/SIA). The development of a miniaturised membrane unit, carrying a single hollow fibre, was devised and integrated with capillary-LC and with CE. An easy connection between membrane based liquid-liquid extraction and GC has been proposed. Extending the applicability of non-porous membrane extractions, antibodies have been used for selective recognition and trapping.
|Award date||2000 Oct 13|
|Publication status||Published - 2000|
Bibliographical noteDefence details
Place: Hörsal A at the Chemical Center
Name: Majors, Ron E.
Affiliation: Hewlett Packard, Delaware, USA
Article: I. Sample Preparation Using a Miniaturized Supported Liquid Membrane Device Connected On-line to Packed Capillary Liquid ChromatographyE. Thordarson; S. Pálmarsdóttir; L. Mathiasson and J. Å. JönssonAnal. Chem. 1996, 68, 2559-2563II. Miniaturized supported liquid membrane device for selective on-line enrichment of basic drugs in plasma combined with capillary zone electrophoresisS. Pálmarsdóttir; E. Thordarson; L-E. Edholm; J. Å. Jönsson; L. MathiassonAnal. Chem. 1997, 69, 1732-1737III. Microporous membrane liquid-liquid extraction coupled on-line with normal-phase liquid chromatography for the determination of cationic surfactants in river and waste-waterJ. Norberg, E. Thordarson, L. Mathiasson, J. Å. JönssonJ. Chromatogr. 2000, 869, 523-529IV. Automated liquid membrane extraction for high-performance liquid chromatography of Ropivacaine metabolites in urineJ. Å. Jönsson, M. Andersson, C. Melander, J. Norberg, E. Thordarson, L. MathiassonJ. Chromatogr. 2000, 870, 151-157V. Extracting Syringe-connecting sample preparation and gas chromatographyJ. Norberg and E. ThordarsonAnalyst, 2000, 125, 673-676VI. Automated Extraction of BTEX from urine samples utilising the Extracting Syringe: Screening of GC Injection ParametersE. Thordarson, S. Bergström, A. Mie, J. Å. Jönsson, J. NorbergManuscriptVII. Immunologic Trapping in Supported Liquid Membrane ExtractionE. Thordarson, J. Å. Jönsson, J. EmnéusAnal. Chem., accepted
The information about affiliations in this record was updated in December 2015.
The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)
Subject classification (UKÄ)
- Analytical Chemistry
- extracting syringe
- micro-porous membrane liquid-liquid extraction
- polymeric membrane extraction
- Non-porous membrane extraction
- supported liquid extraction
- Analytical chemistry
- Analytisk kemi