NMR solution structure of calerythrin, an EF-hand calcium-binding protein from Saccharopolyspora erythraea

H Tossavainen, P Permi, A Annila, I Kilpelainen, Torbjörn Drakenberg

Research output: Contribution to journalArticlepeer-review

21 Citations (SciVal)

Abstract

The structure of calerythrin, a prokaryotic 20 kDa calcium-binding protein has been determined by solution NMR spectroscopy. Distance, dihedral angle, J coupling, secondary chemical shift, residual dipolar coupling and radius of gyration restraints reveal four EF-hand motifs arranged in a compact globular structure. A tight turn in the middle of the amino acid sequence brings the two halves, each comprising a pair of EF-hands, close together. The structural similarity between calerythrin and the eukaryotic sarcoplasmic calcium-binding proteins is notable.
Original languageEnglish
Pages (from-to)2505-2512
JournalEuropean Journal of Biochemistry
Volume270
Issue number11
DOIs
Publication statusPublished - 2003

Subject classification (UKÄ)

  • Physical Chemistry

Keywords

  • calcium-binding
  • calerythrin
  • NMR
  • structure

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