Novel Vinylpyridine Based Cationic MIP Monoliths for Enantiomer Separation in CEC

Peter Spégel, Leif Schweitz, Lars I. Andersson, Staffan Nilsson

Research output: Contribution to journalArticlepeer-review

22 Citations (SciVal)

Abstract

Cationic vinylpyridine based molecularly imprinted polymer monoliths were, for the first time, applied to enantiomer separation using capillary electrochromatography. In order to map the synthesis conditions under which superporous monoliths are obtained, capillary columns were prepared by an in situ polymerization technique using varying monomer and porogen compositions. Both electrolyte pH and percentage of the organic modifier acetonitrile were found to affect the electrochromatographic behaviour on such columns. An interesting observation is that the electroosmotic flow changed direction from cathodic at high pH to anodic at low pH of the electrolyte, opening up the opportunity to manipulate the flow. This is attributed to the vinylpyridine based MIP becomes protonated and positively charged at low pH, whereas it is uncharged at high pH where instead negatively charged silanol groups drive the electroosmotic flow. Improved enantiomer resolution, as well as increased retention, was observed when the concentration of acetonitrile in the electrolyte decreased, indicating a significant element of hydrophobic effects in the molecular recognition of the imprinted enantiomer. Enantiomer separation of the non-steroidal anti-inflammatory drug ibuprofen was studied and efficiencies as high as 30,000 plates per meter with an asymmetry factor below 4 were obtained for the last eluting imprinted enantiomer. These values are better than that obtained generally using MIP based chromatography.
Original languageEnglish
Pages (from-to)277-285
JournalChromatographia
Volume69
Issue number3-4
DOIs
Publication statusPublished - 2009

Bibliographical note

The information about affiliations in this record was updated in December 2015.
The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)

Subject classification (UKÄ)

  • Analytical Chemistry

Keywords

  • Capillary electrochromatography
  • Molecularly imprinted polymer
  • Monolith
  • Vinylpyridine
  • Ibuprofen

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