On Lactobacillus plantarum 299v, bacterial translocation and intestinal permeability.

Peter Mangell

On Lactobacillus plantarum 299v, bacterial translocation and intestinal permeability.

Peter Mangell

Surgery

Research output: Thesis › Doctoral Thesis (compilation)

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Abstract

The gastrointestinal tract harbours a huge load of bacteria which exerts important biological functions to maintain health. In certain conditions the relation between commensal bacteria and pathogens may be set off balance, with an increase in number of pathogens and risk of bacterial translocation (BT). Disease and trauma may also render the intestinal barrier dysfunctional, inducing BT and increased permeability. Probiotic bacteria, i.e. living bacteria which, when ingested, exerts beneficial effects on the host, have been found to stabilize the intestinal mucosa and affect the intestinal microflora. The aim of this thesis was to study the effects of Lactobacillus plantarum 299v in relation to intestinal permeability and BT.

In study I L. plantarum 299v normalized E. coli-induced permeability in distal ileum of rats. This effect was achieved with a more continuous supply of L. plantarum 299v, rather than intermittent or acute pretreatment.

Study II showed that pretreatment with L. plantarum 299v prevented BT in rats rendered septic with LPS. The protective effect seemed to be dependent on the ability of L. plantarum 299v to adhere to intestinal mucosa, indicating competitive inhibition as a possible mechanism behind prevention of translocation. Moreover, treatment with prebiotics, without a probioticum, did not prevent translocation.

In study III LPS-induced intestinal barrier dysfunction on rats was found to be regulated by P-selectin-dependent leukocyte recruitment. With anti-P-selectin antibody intestinal permeability was attenuated, as well as leukocyte rolling and adhesion. This indicates anti-P-selectin treatment as a mean of ameliorating barrier dysfunction in sepsis.

Finally, in study IV, it was found that high doses of L. plantarum 299v given to patients undergoing colon surgery were not able to reduce translocation of selected enteric bacteria but seemed to have a stimulatory effect on bacterial load in the colon. Further, L. plantarum 299v could be given to patients with malignancy without risk of tumour proliferation.

Taken together, the findings herein indicates a potential of L. plantarum 299v pretreatment in reducing BT and intestinal permeability and warrants further studies in the clinical setting.

Original language	English
Qualification	Doctor
Awarding Institution	Surgery
Supervisors/Advisors	Jeppsson, Bengt, Supervisor
Award date	2007 Feb 8
Publisher	Department of Surgery, Malmö University Hospital, Lund University
ISBN (Print)	91-85559-85-7
Publication status	Published - 2007

Bibliographical note

Defence details

Date: 2007-02-08
Time: 09:00
Place: Lecture hall, Dept of Surgery, Malmö University Hospital, entrance 42

External reviewer(s)

Name: Söderholm, Johan
Title: Associate professor
Affiliation: Dept or Surgery, University of Linköping

---

<div class="article_info">P Mangell, P Nejdfors, M Wang, S Ahrné, B Weström, H Thorlacius and B Jeppsson. <span class="article_issue_date">2002</span>. <a href="javascript:downloadFile(547952)" class="article_link">Lactobacillus plantarum 299v Inhibits Escherichia coli-Induced Intestinal Permeability.</a> <span class="journal_series_title">Dig Dis Sci</span>, <span class="journal_volume">vol 47</span> <span class="journal_pages">pp 511-16</span>.</div>
<div class="article_info">P Mangell, P Lennernäs, M Wang, C Olsson, S Ahrné, G Molin, H Thorlacius and B Jeppsson. <span class="article_issue_date">2006</span>. <a href="javascript:downloadFile(547953)" class="article_link">Adhesive Capability of Lactobacillus plantarum 299v is Important for Preventing Bacterial Translocation in Endotoxaemic Rats.</a> <span class="journal_series_title">APMIS</span>, <span class="journal_volume">vol 114</span> <span class="journal_pages">pp 611-8</span>.</div>
<div class="article_info">P Mangell, A Mihaescu, Y Wang, R Schramm, B Jeppsson and H Thorlacius. <span class="article_issue_date">2006</span>. <a href="javascript:downloadFile(547954)" class="article_link">Critical Role of P-selectin-dependent Leukocyte Recruitment in Endotoxin-induced Intestinal Barrier Dysfunction in Mice.</a> <span class="journal_series_title">Infl Res</span>, (accepted)</div>
<div class="article_info">P Mangell, H Thorlacius, I Syk, S Ahrné, G Molin, C Olsson and B Jeppsson. <span class="article_issue_date"></span>. <span class="article_title">L. plantarum 299v to Patients Undergoing Colon Resection – A Randomised Placebo-controlled Study.</span> (manuscript)</div>

Subject classification (UKÄ)

Surgery

Free keywords

bacterial translocation
permeability
intestinal microflora
Kirurgi
orthopaedics
P-selectin
ortopedi
traumatologi
leukocyte recruitment
Lactobacillus plantarum 299v
traumatology
Surgery
colon resection

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Cite this

@phdthesis{d55311a0f3f94662be9bfa2647d0d03d,

title = "On Lactobacillus plantarum 299v, bacterial translocation and intestinal permeability.",

abstract = "The gastrointestinal tract harbours a huge load of bacteria which exerts important biological functions to maintain health. In certain conditions the relation between commensal bacteria and pathogens may be set off balance, with an increase in number of pathogens and risk of bacterial translocation (BT). Disease and trauma may also render the intestinal barrier dysfunctional, inducing BT and increased permeability. Probiotic bacteria, i.e. living bacteria which, when ingested, exerts beneficial effects on the host, have been found to stabilize the intestinal mucosa and affect the intestinal microflora. The aim of this thesis was to study the effects of Lactobacillus plantarum 299v in relation to intestinal permeability and BT. In study I L. plantarum 299v normalized E. coli-induced permeability in distal ileum of rats. This effect was achieved with a more continuous supply of L. plantarum 299v, rather than intermittent or acute pretreatment. Study II showed that pretreatment with L. plantarum 299v prevented BT in rats rendered septic with LPS. The protective effect seemed to be dependent on the ability of L. plantarum 299v to adhere to intestinal mucosa, indicating competitive inhibition as a possible mechanism behind prevention of translocation. Moreover, treatment with prebiotics, without a probioticum, did not prevent translocation. In study III LPS-induced intestinal barrier dysfunction on rats was found to be regulated by P-selectin-dependent leukocyte recruitment. With anti-P-selectin antibody intestinal permeability was attenuated, as well as leukocyte rolling and adhesion. This indicates anti-P-selectin treatment as a mean of ameliorating barrier dysfunction in sepsis. Finally, in study IV, it was found that high doses of L. plantarum 299v given to patients undergoing colon surgery were not able to reduce translocation of selected enteric bacteria but seemed to have a stimulatory effect on bacterial load in the colon. Further, L. plantarum 299v could be given to patients with malignancy without risk of tumour proliferation. Taken together, the findings herein indicates a potential of L. plantarum 299v pretreatment in reducing BT and intestinal permeability and warrants further studies in the clinical setting.",

keywords = "bacterial translocation, permeability, intestinal microflora, Kirurgi, orthopaedics, P-selectin, ortopedi, traumatologi, leukocyte recruitment, Lactobacillus plantarum 299v, traumatology, Surgery, colon resection",

author = "Peter Mangell",

note = "Defence details Date: 2007-02-08 Time: 09:00 Place: Lecture hall, Dept of Surgery, Malm{\"o} University Hospital, entrance 42 External reviewer(s) Name: S{\"o}derholm, Johan Title: Associate professor Affiliation: Dept or Surgery, University of Link{\"o}ping --- <div class={"}article_info{"}>P Mangell, P Nejdfors, M Wang, S Ahrn{\'e}, B Westr{\"o}m, H Thorlacius and B Jeppsson. <span class={"}article_issue_date{"}>2002</span>. <a href={"}javascript:downloadFile(547952){"} class={"}article_link{"}>Lactobacillus plantarum 299v Inhibits Escherichia coli-Induced Intestinal Permeability.</a> <span class={"}journal_series_title{"}>Dig Dis Sci</span>, <span class={"}journal_volume{"}>vol 47</span> <span class={"}journal_pages{"}>pp 511-16</span>.</div> <div class={"}article_info{"}>P Mangell, P Lennern{\"a}s, M Wang, C Olsson, S Ahrn{\'e}, G Molin, H Thorlacius and B Jeppsson. <span class={"}article_issue_date{"}>2006</span>. <a href={"}javascript:downloadFile(547953){"} class={"}article_link{"}>Adhesive Capability of Lactobacillus plantarum 299v is Important for Preventing Bacterial Translocation in Endotoxaemic Rats.</a> <span class={"}journal_series_title{"}>APMIS</span>, <span class={"}journal_volume{"}>vol 114</span> <span class={"}journal_pages{"}>pp 611-8</span>.</div> <div class={"}article_info{"}>P Mangell, A Mihaescu, Y Wang, R Schramm, B Jeppsson and H Thorlacius. <span class={"}article_issue_date{"}>2006</span>. <a href={"}javascript:downloadFile(547954){"} class={"}article_link{"}>Critical Role of P-selectin-dependent Leukocyte Recruitment in Endotoxin-induced Intestinal Barrier Dysfunction in Mice.</a> <span class={"}journal_series_title{"}>Infl Res</span>, (accepted)</div> <div class={"}article_info{"}>P Mangell, H Thorlacius, I Syk, S Ahrn{\'e}, G Molin, C Olsson and B Jeppsson. <span class={"}article_issue_date{"}></span>. <span class={"}article_title{"}>L. plantarum 299v to Patients Undergoing Colon Resection – A Randomised Placebo-controlled Study.</span> (manuscript)</div>",

year = "2007",

language = "English",

isbn = "91-85559-85-7",

series = "Lund University Faculty of Medicine Doctoral Dissertation Series ",

publisher = "Department of Surgery, Malm{\"o} University Hospital, Lund University",

type = "Doctoral Thesis (compilation)",

school = "Surgery",

}

TY - THES

T1 - On Lactobacillus plantarum 299v, bacterial translocation and intestinal permeability.

AU - Mangell, Peter

N1 - Defence details Date: 2007-02-08 Time: 09:00 Place: Lecture hall, Dept of Surgery, Malmö University Hospital, entrance 42 External reviewer(s) Name: Söderholm, Johan Title: Associate professor Affiliation: Dept or Surgery, University of Linköping --- <div class="article_info">P Mangell, P Nejdfors, M Wang, S Ahrné, B Weström, H Thorlacius and B Jeppsson. <span class="article_issue_date">2002</span>. <a href="javascript:downloadFile(547952)" class="article_link">Lactobacillus plantarum 299v Inhibits Escherichia coli-Induced Intestinal Permeability.</a> <span class="journal_series_title">Dig Dis Sci</span>, <span class="journal_volume">vol 47</span> <span class="journal_pages">pp 511-16</span>.</div> <div class="article_info">P Mangell, P Lennernäs, M Wang, C Olsson, S Ahrné, G Molin, H Thorlacius and B Jeppsson. <span class="article_issue_date">2006</span>. <a href="javascript:downloadFile(547953)" class="article_link">Adhesive Capability of Lactobacillus plantarum 299v is Important for Preventing Bacterial Translocation in Endotoxaemic Rats.</a> <span class="journal_series_title">APMIS</span>, <span class="journal_volume">vol 114</span> <span class="journal_pages">pp 611-8</span>.</div> <div class="article_info">P Mangell, A Mihaescu, Y Wang, R Schramm, B Jeppsson and H Thorlacius. <span class="article_issue_date">2006</span>. <a href="javascript:downloadFile(547954)" class="article_link">Critical Role of P-selectin-dependent Leukocyte Recruitment in Endotoxin-induced Intestinal Barrier Dysfunction in Mice.</a> <span class="journal_series_title">Infl Res</span>, (accepted)</div> <div class="article_info">P Mangell, H Thorlacius, I Syk, S Ahrné, G Molin, C Olsson and B Jeppsson. <span class="article_issue_date"></span>. <span class="article_title">L. plantarum 299v to Patients Undergoing Colon Resection – A Randomised Placebo-controlled Study.</span> (manuscript)</div>

PY - 2007

Y1 - 2007

N2 - The gastrointestinal tract harbours a huge load of bacteria which exerts important biological functions to maintain health. In certain conditions the relation between commensal bacteria and pathogens may be set off balance, with an increase in number of pathogens and risk of bacterial translocation (BT). Disease and trauma may also render the intestinal barrier dysfunctional, inducing BT and increased permeability. Probiotic bacteria, i.e. living bacteria which, when ingested, exerts beneficial effects on the host, have been found to stabilize the intestinal mucosa and affect the intestinal microflora. The aim of this thesis was to study the effects of Lactobacillus plantarum 299v in relation to intestinal permeability and BT. In study I L. plantarum 299v normalized E. coli-induced permeability in distal ileum of rats. This effect was achieved with a more continuous supply of L. plantarum 299v, rather than intermittent or acute pretreatment. Study II showed that pretreatment with L. plantarum 299v prevented BT in rats rendered septic with LPS. The protective effect seemed to be dependent on the ability of L. plantarum 299v to adhere to intestinal mucosa, indicating competitive inhibition as a possible mechanism behind prevention of translocation. Moreover, treatment with prebiotics, without a probioticum, did not prevent translocation. In study III LPS-induced intestinal barrier dysfunction on rats was found to be regulated by P-selectin-dependent leukocyte recruitment. With anti-P-selectin antibody intestinal permeability was attenuated, as well as leukocyte rolling and adhesion. This indicates anti-P-selectin treatment as a mean of ameliorating barrier dysfunction in sepsis. Finally, in study IV, it was found that high doses of L. plantarum 299v given to patients undergoing colon surgery were not able to reduce translocation of selected enteric bacteria but seemed to have a stimulatory effect on bacterial load in the colon. Further, L. plantarum 299v could be given to patients with malignancy without risk of tumour proliferation. Taken together, the findings herein indicates a potential of L. plantarum 299v pretreatment in reducing BT and intestinal permeability and warrants further studies in the clinical setting.

AB - The gastrointestinal tract harbours a huge load of bacteria which exerts important biological functions to maintain health. In certain conditions the relation between commensal bacteria and pathogens may be set off balance, with an increase in number of pathogens and risk of bacterial translocation (BT). Disease and trauma may also render the intestinal barrier dysfunctional, inducing BT and increased permeability. Probiotic bacteria, i.e. living bacteria which, when ingested, exerts beneficial effects on the host, have been found to stabilize the intestinal mucosa and affect the intestinal microflora. The aim of this thesis was to study the effects of Lactobacillus plantarum 299v in relation to intestinal permeability and BT. In study I L. plantarum 299v normalized E. coli-induced permeability in distal ileum of rats. This effect was achieved with a more continuous supply of L. plantarum 299v, rather than intermittent or acute pretreatment. Study II showed that pretreatment with L. plantarum 299v prevented BT in rats rendered septic with LPS. The protective effect seemed to be dependent on the ability of L. plantarum 299v to adhere to intestinal mucosa, indicating competitive inhibition as a possible mechanism behind prevention of translocation. Moreover, treatment with prebiotics, without a probioticum, did not prevent translocation. In study III LPS-induced intestinal barrier dysfunction on rats was found to be regulated by P-selectin-dependent leukocyte recruitment. With anti-P-selectin antibody intestinal permeability was attenuated, as well as leukocyte rolling and adhesion. This indicates anti-P-selectin treatment as a mean of ameliorating barrier dysfunction in sepsis. Finally, in study IV, it was found that high doses of L. plantarum 299v given to patients undergoing colon surgery were not able to reduce translocation of selected enteric bacteria but seemed to have a stimulatory effect on bacterial load in the colon. Further, L. plantarum 299v could be given to patients with malignancy without risk of tumour proliferation. Taken together, the findings herein indicates a potential of L. plantarum 299v pretreatment in reducing BT and intestinal permeability and warrants further studies in the clinical setting.

KW - bacterial translocation

KW - permeability

KW - intestinal microflora

KW - Kirurgi

KW - orthopaedics

KW - P-selectin

KW - ortopedi

KW - traumatologi

KW - leukocyte recruitment

KW - Lactobacillus plantarum 299v

KW - traumatology

KW - Surgery

KW - colon resection

M3 - Doctoral Thesis (compilation)

SN - 91-85559-85-7

T3 - Lund University Faculty of Medicine Doctoral Dissertation Series

PB - Department of Surgery, Malmö University Hospital, Lund University

ER -

On Lactobacillus plantarum 299v, bacterial translocation and intestinal permeability.

Abstract

Bibliographical note

Subject classification (UKÄ)

Free keywords

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