Optical projection tomography for rapid whole mouse brain imaging

David Nguyen, Paul J. Marchand, Arielle L. Planchette, Julia Nilsson, Miguel Sison, Jérôme Extermann, Antonio Lopez, Marcin Sylwestrzak, Jessica Sordet-Dessimoz, Anja Schmidt-Christensen, Dan Holmberg, Dimitri Van De Ville, Theo Lasser

    Research output: Contribution to journalArticlepeer-review

    Abstract

    In recent years, three-dimensional mesoscopic imaging has gained significant importance in life sciences for fundamental studies at the whole-organ level. In this manuscript, we present an optical projection tomography (OPT) method designed for imaging of the intact mouse brain. The system features an isotropic resolution of ~50 µm and an acquisition time of four to eight minutes, using a 3-day optimized clearing protocol. Imaging of the brain autofluorescence in 3D reveals details of the neuroanatomy, while the use of fluorescent labels displays the vascular network and amyloid deposition in 5xFAD mice, an important model of Alzheimer’s disease (AD). Finally, the OPT images are compared with histological slices.

    Original languageEnglish
    Article number#301816
    Pages (from-to)5637-5650
    Number of pages14
    JournalBiomedical Optics Express
    Volume8
    Issue number12
    DOIs
    Publication statusPublished - 2017 Dec 1

    Subject classification (UKÄ)

    • Radiology, Nuclear Medicine and Medical Imaging

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