Projects per year
Abstract
In recent years, three-dimensional mesoscopic imaging has gained significant importance in life sciences for fundamental studies at the whole-organ level. In this manuscript, we present an optical projection tomography (OPT) method designed for imaging of the intact mouse brain. The system features an isotropic resolution of ~50 µm and an acquisition time of four to eight minutes, using a 3-day optimized clearing protocol. Imaging of the brain autofluorescence in 3D reveals details of the neuroanatomy, while the use of fluorescent labels displays the vascular network and amyloid deposition in 5xFAD mice, an important model of Alzheimer’s disease (AD). Finally, the OPT images are compared with histological slices.
Original language | English |
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Article number | #301816 |
Pages (from-to) | 5637-5650 |
Number of pages | 14 |
Journal | Biomedical Optics Express |
Volume | 8 |
Issue number | 12 |
DOIs | |
Publication status | Published - 2017 Dec 1 |
Subject classification (UKÄ)
- Radiology and Medical Imaging
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Dive into the research topics of 'Optical projection tomography for rapid whole mouse brain imaging'. Together they form a unique fingerprint.Projects
- 1 Finished
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3D imaging of inflammation and autoimmunity
Schmidt-Christensen, A. (Researcher), Holmberg, D. (PI), Lasser, T. (PI) & Nguyen, D. (Research student)
2009/10/01 → 2020/07/31
Project: Research