Optical projection tomography for rapid whole mouse brain imaging

David Nguyen, Paul J. Marchand, Arielle L. Planchette, Julia Nilsson, Miguel Sison, Jérôme Extermann, Antonio Lopez, Marcin Sylwestrzak, Jessica Sordet-Dessimoz, Anja Schmidt-Christensen, Dan Holmberg, Dimitri Van De Ville, Theo Lasser

Research output: Contribution to journalArticlepeer-review

22 Citations (SciVal)


In recent years, three-dimensional mesoscopic imaging has gained significant importance in life sciences for fundamental studies at the whole-organ level. In this manuscript, we present an optical projection tomography (OPT) method designed for imaging of the intact mouse brain. The system features an isotropic resolution of ~50 µm and an acquisition time of four to eight minutes, using a 3-day optimized clearing protocol. Imaging of the brain autofluorescence in 3D reveals details of the neuroanatomy, while the use of fluorescent labels displays the vascular network and amyloid deposition in 5xFAD mice, an important model of Alzheimer’s disease (AD). Finally, the OPT images are compared with histological slices.

Original languageEnglish
Article number#301816
Pages (from-to)5637-5650
Number of pages14
JournalBiomedical Optics Express
Issue number12
Publication statusPublished - 2017 Dec 1

Subject classification (UKÄ)

  • Radiology, Nuclear Medicine and Medical Imaging


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