Optical projection tomography for rapid whole mouse brain imaging

David Nguyen; Paul J. Marchand; Arielle L. Planchette; Julia Nilsson; Miguel Sison; Jérôme Extermann; Antonio Lopez; Marcin Sylwestrzak; Jessica Sordet-Dessimoz; Anja Schmidt-Christensen; Dan Holmberg; Dimitri Van De Ville; Theo Lasser

doi:10.1364/BOE.8.005637

Optical projection tomography for rapid whole mouse brain imaging

David Nguyen, Paul J. Marchand, Arielle L. Planchette, Julia Nilsson, Miguel Sison, Jérôme Extermann, Antonio Lopez, Marcin Sylwestrzak, Jessica Sordet-Dessimoz, Anja Schmidt-Christensen, Dan Holmberg, Dimitri Van De Ville, Theo Lasser

Swiss Federal Institute of Technology

Research output: Contribution to journal › Article › peer-review

Abstract

In recent years, three-dimensional mesoscopic imaging has gained significant importance in life sciences for fundamental studies at the whole-organ level. In this manuscript, we present an optical projection tomography (OPT) method designed for imaging of the intact mouse brain. The system features an isotropic resolution of ~50 µm and an acquisition time of four to eight minutes, using a 3-day optimized clearing protocol. Imaging of the brain autofluorescence in 3D reveals details of the neuroanatomy, while the use of fluorescent labels displays the vascular network and amyloid deposition in 5xFAD mice, an important model of Alzheimer’s disease (AD). Finally, the OPT images are compared with histological slices.

Original language	English
Article number	#301816
Pages (from-to)	5637-5650
Number of pages	14
Journal	Biomedical Optics Express
Volume	8
Issue number	12
DOIs	https://doi.org/10.1364/BOE.8.005637
Publication status	Published - 2017 Dec 1

Subject classification (UKÄ)

Radiology and Medical Imaging

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10.1364/BOE.8.005637

3D imaging of inflammation and autoimmunity
Schmidt-Christensen, A. (Researcher), Holmberg, D. (PI), Lasser, T. (PI) & Nguyen, D. (Research student)
2009/10/01 → 2020/07/31
Project: Research

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Nguyen, D., Marchand, P. J., Planchette, A. L., Nilsson, J., Sison, M., Extermann, J., Lopez, A., Sylwestrzak, M., Sordet-Dessimoz, J., Schmidt-Christensen, A., Holmberg, D., Van De Ville, D., & Lasser, T. (2017). Optical projection tomography for rapid whole mouse brain imaging. Biomedical Optics Express, 8(12), 5637-5650. Article #301816. https://doi.org/10.1364/BOE.8.005637

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AU - Nguyen, David

AU - Marchand, Paul J.

AU - Planchette, Arielle L.

AU - Nilsson, Julia

AU - Sison, Miguel

AU - Extermann, Jérôme

AU - Lopez, Antonio

AU - Sylwestrzak, Marcin

AU - Sordet-Dessimoz, Jessica

AU - Schmidt-Christensen, Anja

AU - Holmberg, Dan

AU - Van De Ville, Dimitri

AU - Lasser, Theo

PY - 2017/12/1

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AB - In recent years, three-dimensional mesoscopic imaging has gained significant importance in life sciences for fundamental studies at the whole-organ level. In this manuscript, we present an optical projection tomography (OPT) method designed for imaging of the intact mouse brain. The system features an isotropic resolution of ~50 µm and an acquisition time of four to eight minutes, using a 3-day optimized clearing protocol. Imaging of the brain autofluorescence in 3D reveals details of the neuroanatomy, while the use of fluorescent labels displays the vascular network and amyloid deposition in 5xFAD mice, an important model of Alzheimer’s disease (AD). Finally, the OPT images are compared with histological slices.

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Optical projection tomography for rapid whole mouse brain imaging

Abstract

Subject classification (UKÄ)

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3D imaging of inflammation and autoimmunity

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