Optimization of integrated chromatography sequences for purification of biopharmaceuticals

Anton Löfgren; Mikael Yamanee-Nolin; Simon Tallvod; Joaquín G. Fons; Niklas Andersson; Bernt Nilsson

doi:10.1002/btpr.2871

Optimization of integrated chromatography sequences for purification of biopharmaceuticals

Anton Löfgren, Mikael Yamanee-Nolin, Simon Tallvod, Joaquín G. Fons, Niklas Andersson, Bernt Nilsson

Research output: Contribution to journal › Article › peer-review

Abstract

With continued development of integrated and continuous downstream purification processes, tuning and optimization become increasingly complicated with additional parameters and codependent variables over the sequence. This article offers a novel perspective of nonlinear optimization of integrated sequences with regard to individual column sizes, flow rates, and scheduling. The problem setup itself is a versatile tool to be used in downstream design which is demonstrated in two case studies: a four-column integrated sequence and a continuously loaded twin-capture setup with five columns.

Original language	English
Article number	e2871
Journal	Biotechnology Progress
Volume	35
Issue number	6
Early online date	2019 Jun 17
DOIs	https://doi.org/10.1002/btpr.2871
Publication status	Published - 2019 Nov

Subject classification (UKÄ)

Pharmaceutical Chemistry

Free keywords

chromatography
continuous bioprocessing
integrated column sequences
multiple column design
optimization

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10.1002/btpr.2871Licence: CC BY-NC

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AU - Yamanee-Nolin, Mikael

AU - Tallvod, Simon

AU - Fons, Joaquín G.

AU - Andersson, Niklas

AU - Nilsson, Bernt

PY - 2019/11

Y1 - 2019/11

N2 - With continued development of integrated and continuous downstream purification processes, tuning and optimization become increasingly complicated with additional parameters and codependent variables over the sequence. This article offers a novel perspective of nonlinear optimization of integrated sequences with regard to individual column sizes, flow rates, and scheduling. The problem setup itself is a versatile tool to be used in downstream design which is demonstrated in two case studies: a four-column integrated sequence and a continuously loaded twin-capture setup with five columns.

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KW - continuous bioprocessing

KW - integrated column sequences

KW - multiple column design

KW - optimization

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JO - Biotechnology Progress

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Optimization of integrated chromatography sequences for purification of biopharmaceuticals

Abstract

Subject classification (UKÄ)

Free keywords

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Developing integrated downstream processes for next-generation biologics

Computer-aided optimization of complex processes in production systems and urban environments

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