P-fimbriae trigger mucosal responses to Escherichia coli in the human urinary tract

Björn Wullt, Göran Bergsten, Hugh Connell, Piotr Röllano, Negash Gebratsedik, Long Hang, Catharina Svanborg

Research output: Contribution to journalArticlepeer-review

72 Citations (SciVal)

Abstract

Uropathogenic Escherichia coli elicit a host response that determines the severity of urinary tract infection (UTI). Specific adherence mechanisms allow the bacteria to initiate this process by targeting epithelial cells in the urinary tract mucosa. Epidemiological studies show a strong association of P-fimbriae with disease severity, suggesting that adherence mediated by these organelles has a direct effect on mucosal inflammation in vivo. The present study examined the ability of P-fimbriae to induce inflammation in the human urinary tract. Patients were subjected to intravesical inoculation with a non-fimbriated E. coli strain or transformants of this strain expressing P-fimbriae. The inflammatory response was analysed as a function of P-fimbrial expression. The P-fimbriated transformants invariably caused higher interleukin (IL)-8, IL-6 and neutrophil responses in the urinary tract than the ABU strain. Furthermore, loss of P-fimbrial expression in vivo was accompanied by a return to background levels of neutrophils, IL-6 and IL-8 in individual patients. The results demonstrate that the pap sequences confer on a non-fimbriated, avirulent strain the ability to induce a host response in the human urinary tract. P-fimbriae thus fulfil the 'molecular Koch-Henle postulates' linking a single virulence factor to host response induction.

Original languageEnglish
Pages (from-to)255-264
Number of pages10
JournalCellular Microbiology
Volume3
Issue number4
DOIs
Publication statusPublished - 2001 Apr 26

Subject classification (UKÄ)

  • Microbiology in the medical area
  • Urology and Nephrology

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